Application of Capsid Integrity (RT-)qPCR to Assessing Occurrence of Intact Viruses in Surface Water and Tap Water in Japan

Capsid integrity (RT-)qPCR has not too long ago been developed to discriminate between intact types from inactivated types of viruses, however its applicability to figuring out integrity of viruses in ingesting water has remained restricted. On this research, we investigated the applying of capsid integrity (RT-)qPCR utilizing cis-dichlorodiammineplatinum (CDDP) with sodium deoxycholate (SD) pretreatment (SD-CDDP-(RT-)qPCR) to detect intact viruses in floor water and faucet water.

A complete of 63 water samples (floor water, n = 20; faucet water, n = 43) had been collected within the Kanto area in Japan and quantified by typical (RT)-qPCR and SD-CDDP-(RT-)qPCR for pepper gentle mottle virus (PMMoV) and 7 different viruses pathogenic to people (Aichivirus (AiV), noroviruses of genotypes I and II, enterovirus, adenovirus kind 40 and 41, and JC and BK polyomaviruses).

In floor water, PMMoV (100%) was extra continuously detected than different human pathogenic viruses (30%-60%), as decided by typical (RT-)qPCR. SD-CDDP-(RT-)qPCR additionally revealed that intact PMMoV (95%) was extra widespread than intact human pathogenic viruses (20%-45%). Within the faucet water samples, a lot of the goal viruses weren’t detected by typical (RT-)qPCR, aside from PMMoV (9%) and AiV (5%). PMMoV remained optimistic (5%), whereas no AiV was detected when examined by SD-CDDP-(RT-)qPCR, indicating that some PMMoV had an intact capsid, whereas AiV had broken capsids.

The presence of AiV within the absence of PMMoV in faucet water produced from groundwater might display the limitation of PMMoV as a viral indicator in groundwater. Along with being plentiful in floor water, PMMoV was detected in faucet water, together with PMMoV with intact capsids. Thus, the absence of intact PMMoV could also be used to ensure the viral security of faucet water produced from floor water.

Time to act-assessing variations in qPCR analyses in organic nitrogen elimination with examples from partial nitritation/anammox methods

Quantitative PCR (qPCR) is broadly used because the gold customary to quantify microbial neighborhood fractions in environmental microbiology and biotechnology. Benchmarking efforts to make sure the comparability of qPCR information for environmental bioprocesses are nonetheless scarce. Additionally, for partial nitritation/anammox (PN/A) methods systematic investigations are nonetheless lacking, rendering meta-analysis of reported developments and generic insights doubtlessly precarious. We report a baseline investigation of the variability of qPCR-based analyses for microbial communities utilized to PN/A methods.

Spherical-robin testing was carried out for 3 PN/A biomass samples in six laboratories, utilizing the respective in-house DNA extraction and qPCR protocols. The focus of extracted DNA was considerably totally different between labs, ranged between 2.7 and 328 ng mg^-1 moist biomass. The variability among the many qPCR abundance information of various labs was very excessive (1-7 log fold) however differed for various goal microbial guilds. DNA extraction brought about most variation (3-7 log fold), adopted by the primers (1-Three log fold). These insights will information environmental scientists and engineers in addition to remedy plant operators within the interpretation of qPCR information.

Secure Reference Genes for qPCR Evaluation in BM-MSCs Present process Osteogenic Differentiation inside 3D Hyaluronan-Primarily based Hydrogels

Reverse transcription quantitative polymerase chain response (RT-qPCR) allows the monitoring of modifications in cell phenotype through the high-throughput screening of quite a few genes. RT-qPCR is a basic strategy in quite a few analysis fields, together with biomaterials, but little consideration has been given to the potential affect of 3D versus monolayer (2D) cell tradition and to the requirement for a continuing validation of the a number of steps of gene expression evaluation.

The intention of this research is to make use of high-quality RNA to establish probably the most appropriate reference genes for RT-qPCR evaluation through the osteogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BM-MSCs). BM-MSCs are cultured beneath osteogenic situations for 28 days in 2D or inside hyaluronic acid hydrogels (3D). RNA is topic to quality control and is then used to establish probably the most steady reference genes utilizing geNorm, NormFinder, and the ∆Cq methodology.

The impact of the reverse transcriptase is investigated, in addition to the expression of osteogenic-related markers. This research reveals marked variations within the stability of reference genes between 2D (RPLP0/GAPDH) and 3D (OAZ1/PPIA) tradition, suggesting that it’s important to decide on applicable reference genes for 3D osteogenic cell cultures. Thus, an intensive validation beneath particular experimental settings is crucial to acquire significant gene expression outcomes.

Growth of PCR, LAMP and qPCR Assays for the Detection of Aflatoxigenic Strains of Aspergillus flavus and A. parasiticus in Hazelnut

Aspergillus flavus and A. parasiticus are two species in a position to produce aflatoxins in foodstuffs, and specifically in hazelnuts, at harvest and through postharvest part. As not all of the strains of those species are aflatoxin producers, it’s essential to develop methods that may detect aflatoxigenic from not aflatoxigenic strains. Two assays, a LAMP (loop-mediated isothermal amplification) and an actual time PCR with TaqMan^® probe had been designed and validated by way of specificity, sensitivity, reproducibility, and repeatability.

The potential of the strains to supply aflatoxins was measured in vitro and each assays confirmed to be particular for the aflatoxigenic strains of A. flavus and A. parasiticus. The restrict of detection of the LAMP assay was 100-999 picograms of DNA, whereas the qPCR detected 160 femtograms of DNA in hazelnuts. Each methods had been validated utilizing artificially inoculated hazelnuts and naturally contaminated hazelnuts.

Eva QPCR SuperMix Kit
K5052400	Biochain	400 reactions	EUR 316

Pro QPCR SuperMix Kit
K5053200	Biochain	200 reactions	EUR 161

Pro QPCR SuperMix Kit
K5053400	Biochain	400 reactions	EUR 291

Fast Eva QPCR SuperMix Kit
K5052002	Biochain	200 rxn	EUR 188

Power Eva QPCR SuperMix Kit
K5057200	Biochain	200 reactions	EUR 161

Power Eva QPCR SuperMix Kit
K5057400	Biochain	400 reactions	EUR 316

Library Quantification qPCR SuperMix
20-abx098896	Abbexa	EUR 276.00 EUR 493.20	1 ml 5 ml

Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 777.6

Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	EUR 594.00 EUR 895.20	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Pro QPCR SuperMix Kit - ROX premixed
K5056200	Biochain	200 reactions	EUR 161

Pro QPCR SuperMix Kit - ROX premixed
K5056400	Biochain	400 reactions	EUR 291

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	EUR 828.00 EUR 678.00	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems

HyperScript RT SuperMix for qPCR
K1074-100	ApexBio	100 rxn (20 uL/rxn)	EUR 350
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR
K1074-50	ApexBio	50 rxn (20 uL/rxn)	EUR 188
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 1062

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	EUR 710.40 EUR 1228.80	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Fast Pro QPCR SuperMix Kit - ROX premixed
K5058200	Biochain	200 reactions	EUR 188

Fast Pro QPCR SuperMix Kit - ROX premixed
K5058400	Biochain	400 reactions	EUR 316

HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 282

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 861.6

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 282

HiScript III RT SuperMix for qPCR (+g DNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 351.6

HyperScript RT SuperMix for qPCR (with gDNA wiper)
K1174-100	ApexBio	100 rxns	EUR 380
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR (with gDNA wiper)
K1174-50	ApexBio	50 rxns	EUR 220
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 292.8

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01	Vazyme	100 rxns (21 μl/rxn)	EUR 174

Malachite Green Phosphate Assay kit
65-POMG-25H	Gentaur Genprice	2500 tests	EUR 333

PCR SuperMix
20-abx098887	Abbexa	EUR 326.40 EUR 693.60	1 ml 5 ml

PCR SuperMix (+dye)
20-abx098003	Abbexa	EUR 493.20 EUR 226.80 EUR 7408.80 EUR 309.60	15 ml 1 ml 480 ml 5 ml

PCR SuperMix (-dye)
20-abx098002	Abbexa	EUR 493.20 EUR 226.80 EUR 309.60	15 ml 1 ml 5 ml

Plus PCR SuperMix
20-abx098888	Abbexa	EUR 292.80 EUR 560.40	1 ml 5 ml

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

T PCR SuperMix (-dye)
20-abx098005	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

T PCR SuperMix (+dye)
20-abx098006	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	EUR 292.80 EUR 594.00	1 ml 5 ml

Library Amplification SuperMix
20-abx098894	Abbexa	EUR 309.60 EUR 644.40	1 ml 5 ml

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	EUR 493.20 EUR 226.80 EUR 309.60	15 ml 1 ml 5 ml

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 106.8

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 169.2

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

Probe One-Step qRT-PCR SuperMix
20-abx09804220ulSystems	Abbexa	EUR 710.40 EUR 1228.80	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 910.8

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 1028.4

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	EUR 861.60 EUR 693.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

SYBR Green qPCR Master Mix
HY-K0501	MedChemExpress	5 mL (500 rxns)	EUR 315.6

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 878.4

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	EUR 260.40 EUR 427.20	1 ml 5 ml

Probe One-Step qRT-PCR SuperMix (GC Rich)
20-abx09804120ulSystems	Abbexa	EUR 594.00 EUR 895.20	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 693.6

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054004	Biochain	400 rxn	EUR 635

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054200	Biochain	200 reactions	EUR 418

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054400	Biochain	400 reactions	EUR 635

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055004	Biochain	400 rxn	EUR 609

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055200	Biochain	200 reactions	EUR 381

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055400	Biochain	400 reactions	EUR 609

HyperScript First-Strand cDNA Synthesis SuperMix
K1073-100	ApexBio	100 rxn (20 uL/rxn)	EUR 352
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HyperScript First-Strand cDNA Synthesis SuperMix
K1073-50	ApexBio	50 rxn (20 uL/rxn)	EUR 188
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

Genious 2X SYBR Green Fast qPCR Mix
RK21204	Abclonal	1 mL	Ask for price

2x SYBR Green qPCR Master Mix
B21202	Bimake	5 mL	EUR 268.8
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix
B21203	Bimake	25 mL	EUR 1027.2
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054002	Biochain	200 rxn	EUR 418

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055002	Biochain	200 rxn	EUR 380

One-Step gDNA Removal and cDNA Synthesis SuperMix
20-abx09885920ulSystems	Abbexa	EUR 1228.80 EUR 895.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

SYBR Green qPCR Master Mix (No ROX)
HY-K0523	MedChemExpress	5 mL (500 rxns )	EUR 308.4

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
20-abx09802220ulSystems	Abbexa	EUR 1128.00 EUR 828.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522	MedChemExpress	5 mL (500 rxns )	EUR 308.4

SYBR Green qPCR Master Mix (High ROX)
HY-K0521	MedChemExpress	1 mL (100 rxns)	EUR 135.6

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)
20-abx09801520ulSystems	Abbexa	EUR 693.60 EUR 594.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)
20-abx09801720ulSystems	Abbexa	EUR 794.40 EUR 644.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)
RK21205	Abclonal	1 ml	Ask for price

All-in-One First-Strand cDNA Synthesis SuperMix for PCR
abx098023-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 878.4

2x SYBR Green qPCR Master Mix (Low ROX)
B21702	Bimake	5 ml	EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21703	Bimake	25 ml	EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21402	Bimake	5 ml	EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21403	Bimake	25 ml	EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
K1173-100	ApexBio	100 rxns	EUR 380
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
K1173-50	ApexBio	50 rxns	EUR 220
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA.

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-25	ApexBio	25ml	EUR 420

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-5	ApexBio	5ml	EUR 120

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-50	ApexBio	50ml	EUR 840

AceQ qPCR SYBR® Green Master Mix
Q111-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR SYBR® Green Master Mix
Q111-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

Green-2-Go qPCR Mastermix (500X20ul Rxn)
QPCR004-S	Bio Basic	4X1.25ml	EUR 303.25

Gentodenz
19-DENZ-500	Gentaur Genprice	500 g	EUR 416

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)
20-abx098008	Abbexa	EUR 260.40 EUR 427.20	1 ml 5 ml

2X Universal SYBR Green Fast qPCR Mix
RK21203	Abclonal	1 mL	Ask for price

BrightCycle Universal SYBR Green qPCR Mix with UDG
RK21219	Abclonal	5mL	EUR 475

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)
QPCR001-R	Bio Basic	4X1.25ml	EUR 303.25

Ultra SYBR Green qPCR Master Mix (2X, with ROX I)
W2601-1	101Bio	-	Ask for price

Ultra SYBR Green qPCR Master Mix (2X, with ROX I)
W2601-5	101Bio	-	Ask for price

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-25	ApexBio	25ml	EUR 420
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-5	ApexBio	5ml	EUR 120
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-50	ApexBio	50ml	EUR 840
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn)
QPCR003-iC	Bio Basic	4X1.25ml	EUR 303.25

Genorise® 2 x qPCR HotStart Super Mix
GR108001	Genorise Scientific	2.0 ml	EUR 199

Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn)
QPCR006-NODYE	Bio Basic	4X1.25ml	EUR 326.22

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)
QPCR002-L	Bio Basic	4X1.25ml	EUR 303.25

Accuris qMax Green, No Rox qPCR Mix, 100 reactions
PR2000-N-100	Benchmark Scientific	1 PC	EUR 77.7

Accuris qMax Green, No Rox qPCR Mix, 1000 reactions
PR2000-N-1000	Benchmark Scientific	1 PC	EUR 623.8

Accuris qMax Green, No Rox qPCR Mix, 500 reactions
PR2000-N-500	Benchmark Scientific	1 PC	EUR 340.2

Accuris qMax Green, Low Rox qPCR Mix, 100 reactions
PR2000-L-100	Benchmark Scientific	1 PC	EUR 77.7

Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions
PR2000-L-1000	Benchmark Scientific	1 PC	EUR 623.8

Accuris qMax Green, Low Rox qPCR Mix, 500 reactions
PR2000-L-500	Benchmark Scientific	1 PC	EUR 340.2

Accuris qMax Green, High Rox qPCR Mix, 100 reactions
PR2000-H-100	Benchmark Scientific	1 PC	EUR 77.7

Accuris qMax Green, High Rox qPCR Mix, 1000 reactions
PR2000-H-1000	Benchmark Scientific	1 PC	EUR 623.8

Accuris qMax Green, High Rox qPCR Mix, 500 reactions
PR2000-H-500	Benchmark Scientific	1 PC	EUR 340.2

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17274-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17275-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn)
QPCR005-NODYE	Bio Basic	4X1.25ml	EUR 303.25

Accuris qMax Green, No Rox qPCR Mix, sample, 20 reactions
PR2000-N-S	Benchmark Scientific	1 PC	EUR 12.9

Accuris qMax Green, Low Rox qPCR Mix, sample, 20 reactions
PR2000-L-S	Benchmark Scientific	1 PC	EUR 12.9

Accuris qMax Green, High Rox qPCR Mix, sample, 20 reactions
PR2000-H-S	Benchmark Scientific	1 PC	EUR 12.9

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
17270	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
17271	AAT Bioquest	5 mL	EUR 308

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17281-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
17272	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
17273	AAT Bioquest	5 mL	EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*
17274	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*
17275	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
17276	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
17277	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17278	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17279	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
17280	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
17281	AAT Bioquest	5 mL	EUR 308

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
RA910A-1	SBI	20 profiles	EUR 1329.6

Kinesis SuperFlange Ferrule Assembly 1/32 Green
CHR3901	Scientific Laboratory Supplies	EACH	EUR 7.2

Kinesis SuperFlange Ferrule Assembly 1/32 Green
CHR3903	Scientific Laboratory Supplies	PK10	EUR 49.2

Animal Detection Probe qPCR Super PreMix
QV114-EN01	Vazyme	400 rxns	EUR 128

Animal Detection Probe qPCR Super PreMix
QV114-EN02	Vazyme	800 rxns	EUR 230.4

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-372L	MiTeGen	10 x 1,25 ml	EUR 818
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-372S	MiTeGen	2 x 1,25 ml	EUR 205
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

50ml TC Tubes, Conical, 440 units/box
04-5540150	Gentaur Genprice	440 units/box	EUR 85.2

GMP IL4, 50µg
04-GMP-HU-IL4-50UG	Gentaur Genprice	50µg	EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE
04-GSB	Gentaur Genprice	EUR 144.00 EUR 96.00	1L 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU
04-RHIL2-02F01	Gentaur Genprice	1 vial	EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02
04-RHIL2-08F02	Gentaur Genprice	1 vial	EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma
04-RHUGM-CSF-7A10	Gentaur Genprice	300 µg	EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Mouse Anti TNP Immunotoxicity
198-TNPG-1	Gentaur Genprice	100 µL	EUR 469.2

Rye Agar A
765-M1854-500G	Gentaur Genprice	500 g	EUR 82

Rye Agar B
765-M1855-500G	Gentaur Genprice	500 g	EUR 93

Porcine Parvovirus Antibody Elisa Test Kit
767-LSY-30009	Gentaur Genprice	192 Wells/kit	EUR 382

1-Step Polymorphs, Human Cell Separation
71-AN221725	Gentaur Genprice	1	EUR 238.8

QuantiChrom Hemoglobin Assay Kit
65-DIHB-250	Gentaur Genprice	250 tests	EUR 473

Exo-Check Exosome Antibody Array
322-EXO-FBS-50A-1	Gentaur Genprice	100 µg	EUR 469.2

EasyNat Quattro
UC0104	Gentaur Genprice	4-module system	EUR 12000

EasyNat Octo
UC0108	Gentaur Genprice	8-module system	EUR 22000

Monkeypox Virus Real Time PCR Kit
ZD-0076-01	Gentaur Genprice	25 tests/kit	Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02	Gentaur Genprice	25 tests/kit	Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

GMP Recombinant Human Interleukin-4 (IL-4)
GMPhuIL-4-50ug	Gentaur Genprice	50ug	EUR 528

AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn
AZ-2301	Real Time Primers	200 Rxn	EUR 214.8

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn
AZ-2305	Real Time Primers	500 Rxn	EUR 384

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn
AZ-2320	Real Time Primers	2000 Rxn	EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn
AZ-2325	Real Time Primers	5000 Rxn	EUR 2817.6

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn
AZ-2401	Real Time Primers	200 Rxn	EUR 214.8

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn
AZ-2405	Real Time Primers	500 Rxn	EUR 384

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn
AZ-2420	Real Time Primers	2000 Rxn	EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn
AZ-2421	Real Time Primers	5000 Rxn	EUR 2817.6

qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-373L	MiTeGen	10 x 1,25 ml	EUR 818
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-373S	MiTeGen	2 x 1,25 ml	EUR 205
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-375L	MiTeGen	10 x 1,25 ml	EUR 982
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-375S	MiTeGen	2 x 1,25 ml	EUR 246
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-374L	MiTeGen	10 x 1,25 ml	EUR 818
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-374S	MiTeGen	2 x 1,25 ml	EUR 205
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-376L	MiTeGen	10 x 1,25 ml	EUR 982
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-376S	MiTeGen	2 x 1,25 ml	EUR 246
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-377L	MiTeGen	10 x 1,25 ml	EUR 982
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-377S	MiTeGen	2 x 1,25 ml	EUR 246
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

ABScript II One Step SYBR Green RT-qPCR Kit
RK20404	Abclonal	10 RXN	Ask for price

Cell Meterâ„¢ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*
16060-200Tests	AAT Bioquest	200 Tests	EUR 334
Description: Mitochondria are major producers of cellular superoxide.

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*
16060	AAT Bioquest	200 Tests	EUR 334

Green CMFDA
GW6492-1	Glentham Life Sciences	1	EUR 252.7

Green CMFDA
GW6492-25	Glentham Life Sciences	25	EUR 3056

Green CMFDA
GW6492-5	Glentham Life Sciences	5	EUR 895.4

Protonexâ„¢ Green 500
21215-1mg	AAT Bioquest	1 mg	EUR 222
Description: Protonex™ Green dye demonstrated pH-dependent fluorescence.

MitoView Green
70054	Biotium	20X50UG	EUR 271.2
Description: Minimum order quantity: 1 unit of 20X50UG

The qPCR was in a position to detect as few as eight cells of aflatoxigenic Aspergillus in naturally contaminated hazelnut. The mixture of the LAMP assay, which will be carried out in lower than an hour, as screening methodology, with the excessive sensitivity of the qPCR, as affirmation assay, is ready to detect aflatoxigenic strains already in area, serving to to protect the meals security of hazelnuts.