Capsid integrity (RT-)qPCR has not too long ago been developed to discriminate between intact types from inactivated types of viruses, however its applicability to figuring out integrity of viruses in ingesting water has remained restricted. On this research, we investigated the applying of capsid integrity (RT-)qPCR utilizing cis-dichlorodiammineplatinum (CDDP) with sodium deoxycholate (SD) pretreatment (SD-CDDP-(RT-)qPCR) to detect intact viruses in floor water and faucet water.
A complete of 63 water samples (floor water, n = 20; faucet water, n = 43) had been collected within the Kanto area in Japan and quantified by typical (RT)-qPCR and SD-CDDP-(RT-)qPCR for pepper gentle mottle virus (PMMoV) and 7 different viruses pathogenic to people (Aichivirus (AiV), noroviruses of genotypes I and II, enterovirus, adenovirus kind 40 and 41, and JC and BK polyomaviruses).
In floor water, PMMoV (100%) was extra continuously detected than different human pathogenic viruses (30%-60%), as decided by typical (RT-)qPCR. SD-CDDP-(RT-)qPCR additionally revealed that intact PMMoV (95%) was extra widespread than intact human pathogenic viruses (20%-45%). Within the faucet water samples, a lot of the goal viruses weren’t detected by typical (RT-)qPCR, aside from PMMoV (9%) and AiV (5%). PMMoV remained optimistic (5%), whereas no AiV was detected when examined by SD-CDDP-(RT-)qPCR, indicating that some PMMoV had an intact capsid, whereas AiV had broken capsids.
The presence of AiV within the absence of PMMoV in faucet water produced from groundwater might display the limitation of PMMoV as a viral indicator in groundwater. Along with being plentiful in floor water, PMMoV was detected in faucet water, together with PMMoV with intact capsids. Thus, the absence of intact PMMoV could also be used to ensure the viral security of faucet water produced from floor water.
Time to act-assessing variations in qPCR analyses in organic nitrogen elimination with examples from partial nitritation/anammox methods
Quantitative PCR (qPCR) is broadly used because the gold customary to quantify microbial neighborhood fractions in environmental microbiology and biotechnology. Benchmarking efforts to make sure the comparability of qPCR information for environmental bioprocesses are nonetheless scarce. Additionally, for partial nitritation/anammox (PN/A) methods systematic investigations are nonetheless lacking, rendering meta-analysis of reported developments and generic insights doubtlessly precarious. We report a baseline investigation of the variability of qPCR-based analyses for microbial communities utilized to PN/A methods.
Spherical-robin testing was carried out for 3 PN/A biomass samples in six laboratories, utilizing the respective in-house DNA extraction and qPCR protocols. The focus of extracted DNA was considerably totally different between labs, ranged between 2.7 and 328 ng mg-1 moist biomass. The variability among the many qPCR abundance information of various labs was very excessive (1-7 log fold) however differed for various goal microbial guilds. DNA extraction brought about most variation (3-7 log fold), adopted by the primers (1-Three log fold). These insights will information environmental scientists and engineers in addition to remedy plant operators within the interpretation of qPCR information.
Secure Reference Genes for qPCR Evaluation in BM-MSCs Present process Osteogenic Differentiation inside 3D Hyaluronan-Primarily based Hydrogels
Reverse transcription quantitative polymerase chain response (RT-qPCR) allows the monitoring of modifications in cell phenotype through the high-throughput screening of quite a few genes. RT-qPCR is a basic strategy in quite a few analysis fields, together with biomaterials, but little consideration has been given to the potential affect of 3D versus monolayer (2D) cell tradition and to the requirement for a continuing validation of the a number of steps of gene expression evaluation.
The intention of this research is to make use of high-quality RNA to establish probably the most appropriate reference genes for RT-qPCR evaluation through the osteogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BM-MSCs). BM-MSCs are cultured beneath osteogenic situations for 28 days in 2D or inside hyaluronic acid hydrogels (3D). RNA is topic to quality control and is then used to establish probably the most steady reference genes utilizing geNorm, NormFinder, and the ∆Cq methodology.
The impact of the reverse transcriptase is investigated, in addition to the expression of osteogenic-related markers. This research reveals marked variations within the stability of reference genes between 2D (RPLP0/GAPDH) and 3D (OAZ1/PPIA) tradition, suggesting that it’s important to decide on applicable reference genes for 3D osteogenic cell cultures. Thus, an intensive validation beneath particular experimental settings is crucial to acquire significant gene expression outcomes.
Growth of PCR, LAMP and qPCR Assays for the Detection of Aflatoxigenic Strains of Aspergillus flavus and A. parasiticus in Hazelnut
Aspergillus flavus and A. parasiticus are two species in a position to produce aflatoxins in foodstuffs, and specifically in hazelnuts, at harvest and through postharvest part. As not all of the strains of those species are aflatoxin producers, it’s essential to develop methods that may detect aflatoxigenic from not aflatoxigenic strains. Two assays, a LAMP (loop-mediated isothermal amplification) and an actual time PCR with TaqMan® probe had been designed and validated by way of specificity, sensitivity, reproducibility, and repeatability.
The potential of the strains to supply aflatoxins was measured in vitro and each assays confirmed to be particular for the aflatoxigenic strains of A. flavus and A. parasiticus. The restrict of detection of the LAMP assay was 100-999 picograms of DNA, whereas the qPCR detected 160 femtograms of DNA in hazelnuts. Each methods had been validated utilizing artificially inoculated hazelnuts and naturally contaminated hazelnuts.
 Eva QPCR SuperMix Kit |
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K5052400 |
Biochain |
400 reactions |
EUR 320 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Pro QPCR SuperMix Kit |
|
K5053200 |
Biochain |
200 reactions |
EUR 188 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit |
|
K5053400 |
Biochain |
400 reactions |
EUR 299 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Library Quantification qPCR SuperMix |
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20-abx098896 |
Abbexa |
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 Power Eva QPCR SuperMix Kit |
|
K5057200 |
Biochain |
200 reactions |
EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Power Eva QPCR SuperMix Kit |
|
K5057400 |
Biochain |
400 reactions |
EUR 320 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems |
Abbexa |
|
-
100 rxns ×20 ul Systems
-
50 rxns × 20 ul Systems
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) OPTIONAL GREEN FILTER (572NM) |
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GDBL-GREEN |
CORNING |
1/pk |
EUR 1052 |
|
Description: Lab Equipment; Axygen Branded EQ |
 Pro QPCR SuperMix Kit - ROX premixed |
|
K5056200 |
Biochain |
200 reactions |
EUR 188 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
|
K5056400 |
Biochain |
400 reactions |
EUR 299 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Green Two-Step qRT-PCR SuperMix |
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abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 648 |
|
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 Green One-Step qRT-PCR SuperMix |
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20-abx09803820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
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 Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058200 |
Biochain |
200 reactions |
EUR 211 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
|
K5058400 |
Biochain |
400 reactions |
EUR 320 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 HiScript II Q RT SuperMix for qPCR |
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R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
) cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 718 |
|
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 SYBR Green qPCR Master Mix |
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HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 263 |
 Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 885 |
|
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 HiScript II Q Select RT SuperMix for qPCR |
|
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
 2x SYBR Green qPCR Master Mix |
|
B21202 |
Bimake |
5 mL |
EUR 224 |
|
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
|
B21203 |
Bimake |
25 mL |
EUR 856 |
|
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
 PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
|
HS-100-QPCR |
CORNING |
100/pk |
EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
 PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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HS-150-QPCR |
CORNING |
100/pk |
EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
) Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 801 |
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) Green One-Step qRT-PCR SuperMix (GC Rich) |
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20-abx09803920ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
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) HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 293 |
 AceQ qPCR SYBR® Green Master Mix |
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Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
AceQ qPCR SYBR® Green Master Mix |
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Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
) Green-2-Go qPCR Mastermix (500X20ul Rxn) |
|
QPCR004-S |
Bio Basic |
4X1.25ml |
EUR 252.71 |
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) SYBR Green qPCR Master Mix (High ROX) |
|
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 113 |
) SYBR Green qPCR Master Mix (Low ROX) |
|
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 257 |
) SYBR Green qPCR Master Mix (No ROX) |
|
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 257 |
) 2x SYBR Green qPCR Master Mix (High ROX) |
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B21402 |
Bimake |
5 ml |
EUR 224 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
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B21403 |
Bimake |
25 ml |
EUR 856 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) 2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21702 |
Bimake |
5 ml |
EUR 224 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
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B21703 |
Bimake |
25 ml |
EUR 856 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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QPCR001-R |
Bio Basic |
4X1.25ml |
EUR 252.71 |
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) Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
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QPCR003-iC |
Bio Basic |
4X1.25ml |
EUR 252.71 |
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) HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
|
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 244 |
 ig SYBR Green qPCR 2X Master Mix - 500 Reactions |
|
3356 |
Intact Genomics |
1/EA |
EUR 288 |
) Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
|
QPCR002-L |
Bio Basic |
4X1.25ml |
EUR 252.71 |
|
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) Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
|
QPCR006-NODYE |
Bio Basic |
4X1.25ml |
EUR 271.85 |
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 PCR SuperMix |
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20-abx098887 |
Abbexa |
|
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) Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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W2601-1 |
101Bio |
- |
Ask for price |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
|
W2601-5 |
101Bio |
- |
Ask for price |
) Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
|
QPCR005-NODYE |
Bio Basic |
4X1.25ml |
EUR 252.71 |
|
|
 OneScriptcDNA Synthesis SuperMix |
|
G451 |
ABM |
25 x 20 ul reactions |
EUR 89 |
OneScriptcDNA Synthesis SuperMix |
|
G452 |
ABM |
100 x 20 ul reactions |
EUR 141 |
 Evo? cDNA Supermix |
|
M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
|
M1168-25 |
Biovision |
|
EUR 267 |
 Novo? cDNA Supermix |
|
M1169-100 |
Biovision |
|
EUR 441 |
Novo? cDNA Supermix |
|
M1169-25 |
Biovision |
|
EUR 289 |
) PCR SuperMix (-dye) |
|
20-abx098002 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
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|
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) PCR SuperMix (+dye) |
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20-abx098003 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 6174.00
-
EUR 258.00
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 cDNA Synthesis SuperMix |
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20-abx09801420ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
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|
 Plus PCR SuperMix |
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20-abx098888 |
Abbexa |
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 Library Amplification SuperMix |
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20-abx098894 |
Abbexa |
|
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 20 profiles) LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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RA910A-1 |
SBI |
20 profiles |
EUR 1108 |
|
|
 OneScriptPlus cDNA Synthesis SuperMix |
|
G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
|
G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
) T PCR SuperMix (-dye) |
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20-abx098005 |
Abbexa |
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) T PCR SuperMix (+dye) |
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20-abx098006 |
Abbexa |
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) EasyPfu PCR SuperMix (-dye) |
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20-abx098009 |
Abbexa |
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) FastPfu PCR SuperMix (-dye) |
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20-abx098010 |
Abbexa |
|
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 Universal qPCR Mixture |
|
QPCR1000-UNIV |
Bio Basic |
4X1.25ml |
EUR 276.2 |
|
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) PCR SuperMix for PAGE (+dye) |
|
20-abx098004 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
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 MitoView Green |
|
70054 |
Biotium |
20X50UG |
EUR 226 |
|
Description: Minimum order quantity: 1 unit of 20X50UG |
 Bromocresol green |
|
20-abx186394 |
Abbexa |
|
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|
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 Brilliant green |
|
BB0242 |
Bio Basic |
25g |
EUR 56.09 |
|
|
 Leucomalachite green |
|
AG105 |
Unibiotest |
1 mg |
EUR 523 |
Leucomalachite green |
|
AT105 |
Unibiotest |
1mg |
EUR 1368 |
 All-in-One cDNA Synthesis SuperMix |
|
B24403 |
Bimake |
200 ractions |
EUR 456 |
|
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
|
B24408 |
Bimake |
1000 ractions |
EUR 1453 |
|
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
 miRNA First-Strand cDNA Synthesis SuperMix |
|
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 732 |
|
|
 Probe One-Step qRT-PCR SuperMix |
|
20-abx09804220ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
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 Fly First-Strand cDNA Synthesis SuperMix |
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20-abx09885820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
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 qPCR Control Template Kit |
|
G929 |
ABM |
100 rxns |
EUR 148 |
 Lentivirus qPCR Quantification Kit |
|
K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
 Retrovirus qPCR Quantification Kit |
|
K1472-100 |
Biovision |
100 Rxns |
Ask for price |
 QPCR Positive Control Kit |
|
M1126-100 |
Biovision |
|
EUR 403 |
 Neural Lineage qPCR Profiler |
|
RA500A-1 |
SBI |
10 reactions |
EUR 999 |
|
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 Cancer MicroRNA qPCR Array |
|
RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
|
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 Regulatory RNA qPCR Profiler |
|
RA950A-1 |
SBI |
20 rxn |
EUR 880 |
|
|
 Leucomalachite green-HRP |
|
80-1192 |
Fitzgerald |
500 ul |
EUR 137 |
|
Description: Leucomalachite green Conjugate for use in immunoassays |
 Safe-Green™ |
|
G108-G |
ABM |
1.0 ml |
EUR 71 |
 Malachite Green antibody |
|
20-1184 |
Fitzgerald |
100 ul |
EUR 879 |
|
Description: Sheep polyclonal Malachite Green antibody |
 Green Taq Mix |
|
P131-01 |
Vazyme |
5 ml |
EUR 119 |
Green Taq Mix |
|
P131-02 |
Vazyme |
15 ml |
EUR 150 |
Green Taq Mix |
|
P131-03 |
Vazyme |
50 ml |
EUR 248 |
 ViaVac Red/Green |
|
29068 |
Biotium |
100uL |
EUR 261 |
|
Description: Minimum order quantity: 1 unit of 100uL |
 BactoViewâ„¢ Live Green |
|
40102 |
Biotium |
100uL |
EUR 320 |
|
Description: Minimum order quantity: 1 unit of 100uL |
 Leuco- Malachiet green |
|
AG131 |
Unibiotest |
1 mg |
EUR 523 |
 Malachite green oxalate |
|
abx180007-25g |
Abbexa |
25 g |
EUR 272 |
|
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 C.I.Solvent Green 3 |
|
abx185369-25g |
Abbexa |
25 g |
EUR 509 |
|
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 Fast Green FCF |
|
abx082188-5g |
Abbexa |
5 g |
EUR 203 |
|
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 Green Fluorescent Protein |
|
MO22184 |
Neuromics |
100 ul |
EUR 409 |
Green Fluorescent Protein |
|
CH22124 |
Neuromics |
100 ul of 1 mg/ml |
EUR 435 |
 ViSafe Green Gel |
|
S435 |
GeneOn |
500 µl |
EUR 146 |
ViSafe Green Gel |
|
S435L |
GeneOn |
5x500 µl |
EUR 557 |
 Green-DNA Dye |
|
SB2040 |
Bio Basic |
100UL, 100UL |
EUR 67.4 |
|
|
 0.6ML MICROTUBES. GREEN |
|
MCT-060-G |
CORNING |
1000/pk |
EUR 183 |
|
Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
 1.7ML MICROTUBES. GREEN |
|
MCT-175-G |
CORNING |
500/pk |
EUR 116 |
|
Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
Malachite green oxalate |
|
MD0330 |
Bio Basic |
50g |
EUR 57.83 |
|
|
 Accuris qMax Green |
|
PR2000-H-100 |
BenchMark |
1 PC |
EUR 115.24 |
|
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Accuris qMax Green |
|
PR2000-H-1000 |
BenchMark |
1 PC |
EUR 467.74 |
|
|
Accuris qMax Green |
|
PR2000-H-500 |
BenchMark |
1 PC |
EUR 284.31 |
|
|
Accuris qMax Green |
|
PR2000-H-S |
BenchMark |
1 PC |
EUR 73.77 |
|
|
Accuris qMax Green |
|
PR2000-L-100 |
BenchMark |
1 PC |
EUR 115.24 |
|
|
Accuris qMax Green |
|
PR2000-L-1000 |
BenchMark |
1 PC |
EUR 467.74 |
|
|
Accuris qMax Green |
|
PR2000-L-500 |
BenchMark |
1 PC |
EUR 284.31 |
|
|
Accuris qMax Green |
|
PR2000-L-S |
BenchMark |
1 PC |
EUR 73.77 |
|
|
Accuris qMax Green |
|
PR2000-N-100 |
BenchMark |
1 PC |
EUR 113.65 |
|
|
Accuris qMax Green |
|
PR2000-N-1000 |
BenchMark |
1 PC |
EUR 459.76 |
|
|
Accuris qMax Green |
|
PR2000-N-500 |
BenchMark |
1 PC |
EUR 279.53 |
|
|
Accuris qMax Green |
|
PR2000-N-S |
BenchMark |
1 PC |
EUR 72.98 |
|
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) Indocyanine green(Cardiogreen) |
|
E1KS1924 |
EnoGene |
50 mg |
EUR 247 |
 Malachite green Antibody |
|
48363-100ul |
SAB |
100ul |
EUR 333 |
Malachite green Antibody |
|
48363-50ul |
SAB |
50ul |
EUR 239 |
 First-Strand cDNA Synthesis SuperMix for PCR |
|
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 578 |
|
|
) Two-Step RT-PCR SuperMix (12 kb) |
|
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 606 |
|
|
) Two-Step RT-PCR SuperMix (15 kb) |
|
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 759 |
|
|
 BrightGreen 5X qPCR MasterMix-iCycler |
|
MasterMix-5C |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 TaqProbe 5X qPCR MasterMix-ROX |
|
MasterMix-5P |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 TaqProbe 5X qPCR MasterMix-iCycler |
|
MasterMix-5PC |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 TaqProbe 5X qPCR MasterMix-Multiplex |
|
MasterMix-5PM |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 BrightGreen 5X qPCR MasterMix-ROX |
|
MasterMix-5R |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 BrightGreen 2X qPCR MasterMix-iCycler |
|
MasterMix-iC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-iCycler |
|
MasterMix-iC-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
 KiloGreen 2X qPCR MasterMix-iCycler |
|
MasterMix-KC |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
 KiloGreen 2X qPCR MasterMix-ROX |
|
MasterMix-KR |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
 BrightGreen miRNA qPCR MasterMix-iCycler |
|
MasterMix-mC |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
 BrightGreen miRNA qPCR MasterMix-ROX |
|
MasterMix-mR |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
 TaqProbe 2X qPCR MasterMix-ROX |
|
MasterMix-P |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
 TaqProbe 2X qPCR MasterMix-iCycler |
|
MasterMix-PC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
 TaqProbe 2X qPCR MasterMix-Multiplex |
|
MasterMix-PM |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
 BrightGreen 2X qPCR MasterMix-ROX |
|
MasterMix-R |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
|
MasterMix-R-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
 AceQ qPCR Probe Master Mix |
|
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
AceQ qPCR Probe Master Mix |
|
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
) HotTaq Probe qPCR Mix (ROX) |
|
BT11001 |
Bioatlas |
250rxn |
EUR 109 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
) HotTaq Probe qPCR Mix (Capillary) |
|
BT11003 |
Bioatlas |
250rxn |
EUR 109 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
 HotTaq Probe qPCR Universal Mix |
|
BT11004 |
Bioatlas |
250rxn |
EUR 123 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
) HotTaq EvaGreen qPCR Mix (ROX) |
|
BT11101 |
Bioatlas |
250rxn |
EUR 123 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
) HotTaq EvaGreen qPCR Mix (Capillary) |
|
BT11103 |
Bioatlas |
250rxn |
EUR 123 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
 HotTaq EvaGreen qPCR Universal Mix |
|
BT11104 |
Bioatlas |
250rxn |
EUR 138 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
 Methylamp MS-qPCR Fast Kit |
|
P-1028 |
EpiGentek |
200 Reactions |
EUR 496.6 |
|
Description: fast delivery possible |
 RT Master Mix for qPCR |
|
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 291 |
 50ml TC Tubes, Conical, 440 units/box |
|
04-5540150 |
Gentaur Genprice |
440 units/box |
EUR 71 |
 GMP IL4, 50µg |
|
04-GMP-HU-IL4-50UG |
Gentaur Genprice |
50µg |
EUR 483 |
|
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques. |
 SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
|
04-GSB |
Gentaur Genprice |
|
|
|
|
|
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
 rHu IL 2 , 3MIU |
|
04-RHIL2-02F01 |
Gentaur Genprice |
1 vial |
EUR 249 |
|
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
 rHu IL 2 , 3MIU , Lot 200908F02 |
|
04-RHIL2-08F02 |
Gentaur Genprice |
1 vial |
EUR 249 |
|
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
 PRE-GMP rHu GM-CSF, Molgramostim-Leukoma |
|
04-RHUGM-CSF-7A10 |
Gentaur Genprice |
300 µg |
EUR 385 |
|
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD. |
 20 profiles) LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
|
RA900A-1 |
SBI |
20 profiles |
EUR 850 |
|
|
The qPCR was in a position to detect as few as eight cells of aflatoxigenic Aspergillus in naturally contaminated hazelnut. The mixture of the LAMP assay, which will be carried out in lower than an hour, as screening methodology, with the excessive sensitivity of the qPCR, as affirmation assay, is ready to detect aflatoxigenic strains already in area, serving to to protect the meals security of hazelnuts.
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