Capsid integrity (RT-)qPCR has not too long ago been developed to discriminate between intact types from inactivated types of viruses, however its applicability to figuring out integrity of viruses in ingesting water has remained restricted. On this research, we investigated the applying of capsid integrity (RT-)qPCR utilizing cis-dichlorodiammineplatinum (CDDP) with sodium deoxycholate (SD) pretreatment (SD-CDDP-(RT-)qPCR) to detect intact viruses in floor water and faucet water.
A complete of 63 water samples (floor water, n = 20; faucet water, n = 43) had been collected within the Kanto area in Japan and quantified by typical (RT)-qPCR and SD-CDDP-(RT-)qPCR for pepper gentle mottle virus (PMMoV) and 7 different viruses pathogenic to people (Aichivirus (AiV), noroviruses of genotypes I and II, enterovirus, adenovirus kind 40 and 41, and JC and BK polyomaviruses).
In floor water, PMMoV (100%) was extra continuously detected than different human pathogenic viruses (30%-60%), as decided by typical (RT-)qPCR. SD-CDDP-(RT-)qPCR additionally revealed that intact PMMoV (95%) was extra widespread than intact human pathogenic viruses (20%-45%). Within the faucet water samples, a lot of the goal viruses weren’t detected by typical (RT-)qPCR, aside from PMMoV (9%) and AiV (5%). PMMoV remained optimistic (5%), whereas no AiV was detected when examined by SD-CDDP-(RT-)qPCR, indicating that some PMMoV had an intact capsid, whereas AiV had broken capsids.
The presence of AiV within the absence of PMMoV in faucet water produced from groundwater might display the limitation of PMMoV as a viral indicator in groundwater. Along with being plentiful in floor water, PMMoV was detected in faucet water, together with PMMoV with intact capsids. Thus, the absence of intact PMMoV could also be used to ensure the viral security of faucet water produced from floor water.
Time to act-assessing variations in qPCR analyses in organic nitrogen elimination with examples from partial nitritation/anammox methods
Quantitative PCR (qPCR) is broadly used because the gold customary to quantify microbial neighborhood fractions in environmental microbiology and biotechnology. Benchmarking efforts to make sure the comparability of qPCR information for environmental bioprocesses are nonetheless scarce. Additionally, for partial nitritation/anammox (PN/A) methods systematic investigations are nonetheless lacking, rendering meta-analysis of reported developments and generic insights doubtlessly precarious. We report a baseline investigation of the variability of qPCR-based analyses for microbial communities utilized to PN/A methods.

Spherical-robin testing was carried out for 3 PN/A biomass samples in six laboratories, utilizing the respective in-house DNA extraction and qPCR protocols. The focus of extracted DNA was considerably totally different between labs, ranged between 2.7 and 328 ng mg-1 moist biomass. The variability among the many qPCR abundance information of various labs was very excessive (1-7 log fold) however differed for various goal microbial guilds. DNA extraction brought about most variation (3-7 log fold), adopted by the primers (1-Three log fold). These insights will information environmental scientists and engineers in addition to remedy plant operators within the interpretation of qPCR information.
Secure Reference Genes for qPCR Evaluation in BM-MSCs Present process Osteogenic Differentiation inside 3D Hyaluronan-Primarily based Hydrogels
Reverse transcription quantitative polymerase chain response (RT-qPCR) allows the monitoring of modifications in cell phenotype through the high-throughput screening of quite a few genes. RT-qPCR is a basic strategy in quite a few analysis fields, together with biomaterials, but little consideration has been given to the potential affect of 3D versus monolayer (2D) cell tradition and to the requirement for a continuing validation of the a number of steps of gene expression evaluation.
The intention of this research is to make use of high-quality RNA to establish probably the most appropriate reference genes for RT-qPCR evaluation through the osteogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BM-MSCs). BM-MSCs are cultured beneath osteogenic situations for 28 days in 2D or inside hyaluronic acid hydrogels (3D). RNA is topic to quality control and is then used to establish probably the most steady reference genes utilizing geNorm, NormFinder, and the ∆Cq methodology.
The impact of the reverse transcriptase is investigated, in addition to the expression of osteogenic-related markers. This research reveals marked variations within the stability of reference genes between 2D (RPLP0/GAPDH) and 3D (OAZ1/PPIA) tradition, suggesting that it’s important to decide on applicable reference genes for 3D osteogenic cell cultures. Thus, an intensive validation beneath particular experimental settings is crucial to acquire significant gene expression outcomes.
Growth of PCR, LAMP and qPCR Assays for the Detection of Aflatoxigenic Strains of Aspergillus flavus and A. parasiticus in Hazelnut
Aspergillus flavus and A. parasiticus are two species in a position to produce aflatoxins in foodstuffs, and specifically in hazelnuts, at harvest and through postharvest part. As not all of the strains of those species are aflatoxin producers, it’s essential to develop methods that may detect aflatoxigenic from not aflatoxigenic strains. Two assays, a LAMP (loop-mediated isothermal amplification) and an actual time PCR with TaqMan® probe had been designed and validated by way of specificity, sensitivity, reproducibility, and repeatability.
The potential of the strains to supply aflatoxins was measured in vitro and each assays confirmed to be particular for the aflatoxigenic strains of A. flavus and A. parasiticus. The restrict of detection of the LAMP assay was 100-999 picograms of DNA, whereas the qPCR detected 160 femtograms of DNA in hazelnuts. Each methods had been validated utilizing artificially inoculated hazelnuts and naturally contaminated hazelnuts.
Eva QPCR SuperMix Kit |
K5052400 |
Biochain |
400 reactions |
EUR 316 |
Pro QPCR SuperMix Kit |
K5053200 |
Biochain |
200 reactions |
EUR 161 |
Pro QPCR SuperMix Kit |
K5053400 |
Biochain |
400 reactions |
EUR 291 |
Fast Eva QPCR SuperMix Kit |
K5052002 |
Biochain |
200 rxn |
EUR 188 |
Power Eva QPCR SuperMix Kit |
K5057200 |
Biochain |
200 reactions |
EUR 161 |
Power Eva QPCR SuperMix Kit |
K5057400 |
Biochain |
400 reactions |
EUR 316 |
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
|
|
|
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 777.6 |
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 161 |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 291 |
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
Abbexa |
|
- 100 rxns ×20 ul Systems
- 50 rxns × 20 ul Systems
|
|
HyperScript RT SuperMix for qPCR |
K1074-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 350 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
K1074-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 188 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
Green miRNA Two-Step qRT-PCR SuperMix |
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 1062 |
|
Green Two-Step qRT-PCR SuperMix (GC Rich) |
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 961.2 |
|
Green One-Step qRT-PCR SuperMix (GC Rich) |
20-abx09803920ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 188 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 316 |
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
|
HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 351.6 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-100 |
ApexBio |
100 rxns |
EUR 380 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-50 |
ApexBio |
50 rxns |
EUR 220 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 292.8 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 174 |
Malachite Green Phosphate Assay kit |
65-POMG-25H |
Gentaur Genprice |
2500 tests |
EUR 333 |
PCR SuperMix |
20-abx098887 |
Abbexa |
|
|
|
PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 7408.80
-
EUR 309.60
|
|
|
PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
|
|
Plus PCR SuperMix |
20-abx098888 |
Abbexa |
|
|
|
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
|
|
|
T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
|
|
|
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
|
|
|
FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
|
|
|
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
|
|
|
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
|
|
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 727.2 |
|
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 910.8 |
|
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 727.2 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 794.4 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098029-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 1028.4 |
|
Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 315.6 |
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 878.4 |
|
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
20-abx098007 |
Abbexa |
|
|
|
Probe One-Step qRT-PCR SuperMix (GC Rich) |
20-abx09804120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 693.6 |
|
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054004 |
Biochain |
400 rxn |
EUR 635 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 418 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 635 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055004 |
Biochain |
400 rxn |
EUR 609 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 381 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 609 |
HyperScript First-Strand cDNA Synthesis SuperMix |
K1073-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 352 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix |
K1073-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 188 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
Ask for price |
|
2x SYBR Green qPCR Master Mix |
B21202 |
Bimake |
5 mL |
EUR 268.8 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
B21203 |
Bimake |
25 mL |
EUR 1027.2 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054002 |
Biochain |
200 rxn |
EUR 418 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055002 |
Biochain |
200 rxn |
EUR 380 |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
20-abx09885920ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
SYBR Green qPCR Master Mix (No ROX) |
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
20-abx09801620ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
20-abx09802120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
20-abx09802220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
SYBR Green qPCR Master Mix (Low ROX) |
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix (High ROX) |
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
20-abx09801520ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
20-abx09801720ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
RK21205 |
Abclonal |
1 ml |
Ask for price |
|
All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 878.4 |
|
2x SYBR Green qPCR Master Mix (Low ROX) |
B21702 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21402 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper) |
K1173-100 |
ApexBio |
100 rxns |
EUR 380 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper) |
K1173-50 |
ApexBio |
50 rxns |
EUR 220 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-25 |
ApexBio |
25ml |
EUR 420 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-5 |
ApexBio |
5ml |
EUR 120 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-50 |
ApexBio |
50ml |
EUR 840 |
AceQ qPCR SYBR® Green Master Mix |
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
Green-2-Go qPCR Mastermix (500X20ul Rxn) |
QPCR004-S |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA) |
20-abx098008 |
Abbexa |
|
|
|
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
Ask for price |
|
BrightCycle Universal SYBR Green qPCR Mix with UDG |
RK21219 |
Abclonal |
5mL |
EUR 475 |
Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
QPCR001-R |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
W2601-1 |
101Bio |
- |
Ask for price |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
W2601-5 |
101Bio |
- |
Ask for price |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-25 |
ApexBio |
25ml |
EUR 420 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-5 |
ApexBio |
5ml |
EUR 120 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-50 |
ApexBio |
50ml |
EUR 840 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
QPCR003-iC |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
QPCR006-NODYE |
Bio Basic |
4X1.25ml |
EUR 326.22 |
|
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
QPCR002-L |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Accuris qMax Green, No Rox qPCR Mix, 1000 reactions |
PR2000-N-1000 |
Benchmark Scientific |
1 PC |
EUR 623.8 |
Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions |
PR2000-L-1000 |
Benchmark Scientific |
1 PC |
EUR 623.8 |
Accuris qMax Green, Low Rox qPCR Mix, 500 reactions |
PR2000-L-500 |
Benchmark Scientific |
1 PC |
EUR 340.2 |
Accuris qMax Green, High Rox qPCR Mix, 100 reactions |
PR2000-H-100 |
Benchmark Scientific |
1 PC |
EUR 77.7 |
Accuris qMax Green, High Rox qPCR Mix, 1000 reactions |
PR2000-H-1000 |
Benchmark Scientific |
1 PC |
EUR 623.8 |
Accuris qMax Green, High Rox qPCR Mix, 500 reactions |
PR2000-H-500 |
Benchmark Scientific |
1 PC |
EUR 340.2 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
QPCR005-NODYE |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Accuris qMax Green, No Rox qPCR Mix, sample, 20 reactions |
PR2000-N-S |
Benchmark Scientific |
1 PC |
EUR 12.9 |
Accuris qMax Green, Low Rox qPCR Mix, sample, 20 reactions |
PR2000-L-S |
Benchmark Scientific |
1 PC |
EUR 12.9 |
Accuris qMax Green, High Rox qPCR Mix, sample, 20 reactions |
PR2000-H-S |
Benchmark Scientific |
1 PC |
EUR 12.9 |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17270 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17271 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17272 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17273 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17274 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17275 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 308 |
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
RA910A-1 |
SBI |
20 profiles |
EUR 1329.6 |
|
Animal Detection Probe qPCR Super PreMix |
QV114-EN01 |
Vazyme |
400 rxns |
EUR 128 |
Animal Detection Probe qPCR Super PreMix |
QV114-EN02 |
Vazyme |
800 rxns |
EUR 230.4 |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372L |
MiTeGen |
10 x 1,25 ml |
EUR 818 |
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372S |
MiTeGen |
2 x 1,25 ml |
EUR 205 |
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
50ml TC Tubes, Conical, 440 units/box |
04-5540150 |
Gentaur Genprice |
440 units/box |
EUR 85.2 |
GMP IL4, 50µg |
04-GMP-HU-IL4-50UG |
Gentaur Genprice |
50µg |
EUR 579.6 |
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques. |
SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
04-GSB |
Gentaur Genprice |
|
|
|
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
rHu IL 2 , 3MIU |
04-RHIL2-02F01 |
Gentaur Genprice |
1 vial |
EUR 298.8 |
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
rHu IL 2 , 3MIU , Lot 200908F02 |
04-RHIL2-08F02 |
Gentaur Genprice |
1 vial |
EUR 298.8 |
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated. |
PRE-GMP rHu GM-CSF, Molgramostim-Leukoma |
04-RHUGM-CSF-7A10 |
Gentaur Genprice |
300 µg |
EUR 462 |
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD. |
Porcine Parvovirus Antibody Elisa Test Kit |
767-LSY-30009 |
Gentaur Genprice |
192 Wells/kit |
EUR 382 |
1-Step Polymorphs, Human Cell Separation |
71-AN221725 |
Gentaur Genprice |
1 |
EUR 238.8 |
Exo-Check Exosome Antibody Array |
322-EXO-FBS-50A-1 |
Gentaur Genprice |
100 µg |
EUR 469.2 |
Monkeypox Virus Real Time PCR Kit |
ZD-0076-01 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
ZD-0076-02 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
GMP Recombinant Human Interleukin-4 (IL-4) |
GMPhuIL-4-50ug |
Gentaur Genprice |
50ug |
EUR 528 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
AZ-2301 |
Real Time Primers |
200 Rxn |
EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
AZ-2305 |
Real Time Primers |
500 Rxn |
EUR 384 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
AZ-2320 |
Real Time Primers |
2000 Rxn |
EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
AZ-2325 |
Real Time Primers |
5000 Rxn |
EUR 2817.6 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
AZ-2401 |
Real Time Primers |
200 Rxn |
EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
AZ-2405 |
Real Time Primers |
500 Rxn |
EUR 384 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
AZ-2420 |
Real Time Primers |
2000 Rxn |
EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
AZ-2421 |
Real Time Primers |
5000 Rxn |
EUR 2817.6 |
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-373L |
MiTeGen |
10 x 1,25 ml |
EUR 818 |
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-373S |
MiTeGen |
2 x 1,25 ml |
EUR 205 |
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-375L |
MiTeGen |
10 x 1,25 ml |
EUR 982 |
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-375S |
MiTeGen |
2 x 1,25 ml |
EUR 246 |
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-374L |
MiTeGen |
10 x 1,25 ml |
EUR 818 |
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-374S |
MiTeGen |
2 x 1,25 ml |
EUR 205 |
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-376L |
MiTeGen |
10 x 1,25 ml |
EUR 982 |
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-376S |
MiTeGen |
2 x 1,25 ml |
EUR 246 |
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-377L |
MiTeGen |
10 x 1,25 ml |
EUR 982 |
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-377S |
MiTeGen |
2 x 1,25 ml |
EUR 246 |
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
ABScript II One Step SYBR Green RT-qPCR Kit |
RK20404 |
Abclonal |
10 RXN |
Ask for price |
|
Cell Meterâ„¢ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence* |
16060-200Tests |
AAT Bioquest |
200 Tests |
EUR 334 |
|
Description: Mitochondria are major producers of cellular superoxide. |
Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence* |
16060 |
AAT Bioquest |
200 Tests |
EUR 334 |
Protonexâ„¢ Green 500 |
21215-1mg |
AAT Bioquest |
1 mg |
EUR 222 |
|
Description: Protonex™ Green dye demonstrated pH-dependent fluorescence. |
MitoView Green |
70054 |
Biotium |
20X50UG |
EUR 271.2 |
Description: Minimum order quantity: 1 unit of 20X50UG |
The qPCR was in a position to detect as few as eight cells of aflatoxigenic Aspergillus in naturally contaminated hazelnut. The mixture of the LAMP assay, which will be carried out in lower than an hour, as screening methodology, with the excessive sensitivity of the qPCR, as affirmation assay, is ready to detect aflatoxigenic strains already in area, serving to to protect the meals security of hazelnuts.
Leave a Comment