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Application of Capsid Integrity (RT-)qPCR to Assessing Occurrence of Intact Viruses in Surface Water and Tap Water in Japan
Capsid integrity (RT-)qPCR has not too long ago been developed to discriminate between intact types from inactivated types of viruses, however its applicability to figuring out integrity of viruses in … Continue Reading →
Prevalence of Histidine Decarboxylase Genes of Gram-Positive Bacteria in Surströmming as Revealed by qPCR
Histamine is a degradation product of the bacterial decarboxylation of the amino acid histidine; such exercise is set by histidine decarboxylase encoded by a gene cluster, carried by some Gram-positive … Continue Reading →
Selection and optimization of reference genes for RT-qPCR normalization: A case study in Solanum lycopersicum exposed to UV-B
Quantitative RT- PCR is without doubt one of the most typical strategies to check gene expression in response to emphasize. Subsequently, it’s essential to have appropriate reference genes (RGs) for … Continue Reading →
Comparison of qPCR and Metabarcoding Methods as Tools for the Detection of Airborne Inoculum of Forest Fungal Pathogens
Forest ailments brought on by invasive fungal pathogens have gotten extra frequent, generally with dramatic penalties to forest ecosystems. The event of early detection methods is critical for environment friendly … Continue Reading →
Analysis of the cox1 gene in Echinococcus granulosus from sheep in northeast Iran using PCR high-resolution melting (qPCR-HRM) curve analysis
Echinococcus granulosus, the etiologic agent of echinococcosis, is among the most vital zoonotic helminthes worldwide. Data of E. granulosus species and genotypes has vital implications for epidemiology, management, and prevention … Continue Reading →