Prevalence of Histidine Decarboxylase Genes of Gram-Positive Bacteria in Surströmming as Revealed by qPCR

Histamine is a degradation product of the bacterial decarboxylation of the amino acid histidine; such exercise is set by histidine decarboxylase encoded by a gene cluster, carried by some Gram-positive micro organism, that features the hdcA gene. On this examine, the presence of the hdcA gene in ready-to-eat surströmming samples collected from three producers primarily based in Sweden was straight assessed through qPCR evaluation for the very first time.

Samples from producer A confirmed hdcA common gene abundance of 6.67 ± 0.13 Log cells/gene copies g-1; in samples from producer B the common worth attested at 5.56 ± 0.06 Log cells/gene copies g-1, whereas for samples of producer C hdcA common gene abundance attested at 5.30 ± 0.08 Log cells/gene copies g-1. ANOVA confirmed a considerably larger common hdcA gene copy quantity in samples from producer A, whereas no vital variations had been seen between common values of hdcA gene copy numbers detected in samples from producer B and C. The hdcA gene copies detected within the current examine might give an estimation of the load of potential histamine-producing micro organism in surströmming.

Decreased ribosomal DNA transcription within the prefrontal cortex of suicide victims: consistence of recent molecular RT-qPCR findings with earlier morphometric information from AgNOR-stained pyramidal neurons

Prefrontal cortical areas play a key function in behavioural regulation, which is profoundly disturbed in suicide. The examine was carried out on frozen cortical samples from the anterior cingulate cortex (dorsal and ventral components, ACd and ACv), the orbitofrontal cortex (OFC), and the dorsolateral cortex (DLC) obtained from 20 suicide completers (predominantly violent) with unknown psychiatric prognosis and 21 non-suicidal controls. The relative stage of ribosomal RNA (rRNA) as a marker of the transcriptional exercise of ribosomal DNA (rDNA) was evaluated bilaterally in prefrontal areas talked about above (i.e. in eight areas of curiosity, ROIs) by reverse transcription and quantitative polymerase chain response (RT-qPCR).

The general statistical evaluation revealed a lower in rDNA exercise in suicide victims versus controls, significantly in male topics. Additional ROI-specific put up hoc analyses revealed a big lower on this exercise in suicides in comparison with non-suicides in 5 ROIs. This impact was accentuated within the ACv, the place it was noticed bilaterally. Our findings counsel that decreased rDNA transcription within the prefrontal cortex performs an necessary function in suicide pathogenesis and corresponds with our earlier morphometric analyses of AgNOR-stained neurons.

Comparability of Bisulfite Pyrosequencing and Methylation-Particular qPCR for Methylation Evaluation

Completely different methodological approaches can be found to evaluate DNA methylation biomarkers. On this examine, we evaluated two sodium bisulfite conversion-dependent strategies, specifically pyrosequencing and methylation-specific qPCR (MS-qPCR), with the intention of measuring the closeness of settlement of methylation values between these two strategies and its impact when setting a cut-off.

Prevalence of Histidine Decarboxylase Genes of Gram-Positive Bacteria in Surströmming as Revealed by qPCR

Methylation of tumor suppressor gene p16/INK4A was evaluated in 80 lung most cancers sufferers from which cytological lymph node samples had been obtained. Cluster analyses had been used to ascertain methylated and unmethylated teams for every technique. Settlement and concordance between pyrosequencing and MS-qPCR was evaluated with Pearson’s correlation, Bland-Altman, Cohen’s kappa index and ROC curve analyses. Primarily based on these analyses, cut-offs had been derived for MS-qPCR.

A suitable correlation (Pearson’s R2 = 0.738) was discovered between pyrosequencing (PYRmean) and MS-qPCR (NMP; normalized methylation proportion), offering related medical outcomes when categorizing information as binary utilizing cluster evaluation. In comparison with pyrosequencing, MS-qPCR tended to underestimate methylation for values between Zero and 15%, whereas for methylation >30% overestimation was noticed.

The estimated cut-off for MS-qPCR information primarily based on cluster evaluation, kappa-index settlement and ROC curve evaluation had been a lot decrease than that derived from pyrosequencing. In conclusion, our outcomes point out that independently of the strategy used for estimating the cut-off, the methylation proportion obtained via MS-qPCR is decrease than that calculated for pyrosequencing. These variations in information and subsequently within the cut-off ought to be examined when utilizing methylation biomarkers within the medical observe.

The results of dithiothreitol (DTT) on fluorescent qPCR dyes

DNA extractions of semen samples generally make the most of dithiothreitol (DTT) to scale back and disrupt disulfide bonds. Though conventional extraction strategies take away DTT earlier than downstream analyses, the forensic DNA neighborhood has lately explored Y-screening, direct amplification, and direct cell lysis assays that omit purification however make use of decreasing brokers to lyse spermatozoa. This examine examined the influence of residual DTT on downstream processes involving fluorescent dyes.

Quantification utilizing Investigator® Quantiplex HYres revealed a big improve within the male DNA yield (p = 0.00056) and a >150,000,000-fold improve within the male:human DNA ratio when DTT remained in extracts versus when it was filtered out utilizing a conventional purification technique. When DTT was current with Quantifiler™ Trio, the true imply DNA yield for the massive autosomal goal considerably elevated (p = 0.038) and the common reported DNA yields elevated 1.1-fold, >9.5-fold, and 1.3-fold for the small autosomal, giant autosomal, and male targets, respectively.

DTT-spiked DNA requirements from each kits had been impacted equally to samples with residual DTT, demonstrating that noticed results had been associated to DTT and never the extraction technique. This examine corroborates different stories that DTT adversely impacts a number of dyes (e.g., Cy5, Quasar 670, SYBR Inexperienced I, TMR, and Mustang Purple® ).

Pro QPCR SuperMix Kit

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Green qPCR SuperMix

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Pro QPCR SuperMix Kit - ROX premixed

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QCell-Eva One-Step qRT-PCR SuperMix Kit

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QCell-Eva One-Step qRT-PCR SuperMix Kit

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Fast Pro QPCR SuperMix Kit - ROX premixed

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Fast Pro QPCR SuperMix Kit - ROX premixed

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Top Green qPCR SuperMix (+Dye II)

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Tip Green qPCR SuperMix (+Dye II)

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HiScript II Q RT SuperMix for qPCR

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HiScript II Q Select RT SuperMix for qPCR

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HiScript III RT SuperMix for qPCR (+g DNA wiper)

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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
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Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

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HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
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PCR SuperMix

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  • EUR 272.00
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OneScriptcDNA Synthesis SuperMix

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EUR 89

OneScriptcDNA Synthesis SuperMix

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EUR 141

PCR SuperMix (-dye)

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PCR SuperMix (+dye)

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cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
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  • 100 rxns × 20 ul Systems
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Plus PCR SuperMix

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Library Amplification SuperMix

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  • EUR 258.00
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Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
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Novo? cDNA Supermix

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Novo? cDNA Supermix

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EUR 289

qPCR Control Template Kit

G929 100 rxns
EUR 148

QPCR Positive Control Kit

M1126-100
EUR 403

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns Ask for price

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

COLLECTION CONTAINER 1L, EVA FILM

91-200-01 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 2L, EVA FILM

91-200-02 1/pk
EUR 88
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-05 1/pk
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COLLECTION CONTAINER 10L, EVA FILM

91-200-10 1/pk
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COLLECTION CONTAINER 20L, EVA FILM

91-200-20 1/pk
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COLLECTION CONTAINER 10L, EVA FILM

91-200-36 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-39 1/pk
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COLLECTION CONTAINER 1L, EVA FILM

91-200-42 1/pk
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91-200-43 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-45 1/pk
EUR 126
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-47 1/pk
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COLLECTION CONTAINER 50L, EVA FILM

91-200-48 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 100L, EVA FILM

91-200-82 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

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OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

T PCR SuperMix (-dye)

20-abx098005
  • EUR 203.00
  • EUR 342.00
  • 1 ml
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T PCR SuperMix (+dye)

20-abx098006
  • EUR 203.00
  • EUR 342.00
  • 1 ml
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EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 203.00
  • EUR 342.00
  • 1 ml
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FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 244.00
  • EUR 495.00
  • 1 ml
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Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

COLLECTION CONTAINER 500 ML, EVA FILM

91-200-41 1/pk
EUR 69
Description: Flexible Containers; Bioprocess Bags and Parts

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

igScript RT-qPCR Kit - 50 Reactions

4513 1/EA
EUR 248

igScript RT-qPCR Kit - 200 Reactions

4515 1/EA
EUR 716

Coronavirus Rapid RT-qPCR Detection Kit

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EUR 551

Adeno-Associated Virus qPCR Quantification Kit

K1473-100 100 Rxns
EUR 348

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

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EUR 1110
  • Category: Cas9

Protein Eva-1 Homolog C (EVA1C) Antibody

20-abx302696
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
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  • 1 mg
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Neural Lineage qPCR Profiler

RA500A-1 10 reactions
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  • Category: Lineage Profilers

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172
  • Category: MicroRNA Tools

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 880
  • Category: Long Non-coding RNA Tools

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 376
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 572
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CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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  • Category: Cas9

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CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

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CASLV120PA-KIT 1 Kit
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  • Category: Cas9

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  • Category: Cas9

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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  • Category: Cas9

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  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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  • Category: Cas9

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  • Category: Cas9

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  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

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  • Category: Cas9

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  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

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All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

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EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

miRNA First-Strand cDNA Synthesis SuperMix

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EUR 732
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Green Two-Step qRT-PCR SuperMix

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Green One-Step qRT-PCR SuperMix

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  • EUR 495.00
  • EUR 746.00
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Probe One-Step qRT-PCR SuperMix

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  • EUR 592.00
  • EUR 1024.00
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Fly First-Strand cDNA Synthesis SuperMix

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  • EUR 718.00
  • EUR 578.00
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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 10 x 1,25 ml
EUR 1719

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 20 reactions
EUR 331
  • Category: PiggyBac Technology

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-iCycler

MasterMix-5PC 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex

MasterMix-5PM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex

MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 646

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 578
  • Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 606
  • Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 759
  • Shipped within 5-10 working days.

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 885
  • Shipped within 5-10 working days.

igScript One Step RT-qPCR Kit - 100 Reactions

4214 1/EA
EUR 253

igScript One Step RT-qPCR Kit - 500 Reactions

4218 1/EA
EUR 773

General, DTT causes inaccurate portions and, consequently, inaccurate calculated male:feminine ratios when used at the side of these kits. Thus, implementation of newer direct-to-PCR assays incorporating DTT ought to both be prevented or used solely with fastidiously evaluated, appropriate dyes.

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