Selection and optimization of reference genes for RT-qPCR normalization: A case study in Solanum lycopersicum exposed to UV-B

Quantitative RT- PCR is without doubt one of the most typical strategies to check gene expression in response to emphasize. Subsequently, it’s essential to have appropriate reference genes (RGs) for outcome normalization. Though a number of experiences describe UV-B-modulated gene expression in Solanum lycopersicum, there aren’t any appropriate RGs recognized till now.

The goal of this work was to guage the suitability of seven conventional genes: actin (ACT), tubulin (TUB), ubiquitin (UBI), glyceraldehyde- Three phosphate dehydrogenase (GAPDH), elongation issue 1α (EF1α), phosphatase 2A catalytic subunit (PP2A) and GAGA binding transcriptional activator (GAGA); and two non-traditional genes: thioredoxin h1 (TRX h1) and UV-B RESISTANCE LOCUS 8 (UVR8), as candidate RGs for his or her potential use as dependable inner controls in leaves, stems and roots of tomato seedlings uncovered to acute and persistent UV-B.

The steadiness of those genes expression was evaluated utilizing 5 statistical algorithms: geNorm, NormFinder, BestKeeper, Delta Ct and ANOVA. Contemplating the excellent stability rating, we suggest ACT+TUB as the perfect pair of RGs for leaves, PP2A+GAPDH+TRX h1 for stems and TUB+UVR8 for roots. The reliability of the chosen RGs for every tissue was verified amplifying tomato chalcone synthase 1 (CHS1) and cyclobutane pyrimidine dimer (CPD) photolyase (PHR1-LIKE). Below UV-B therapy, CHS1 was upregulated in leaves, stems and roots whereas PHR1-LIKE was solely upregulated in leaves and stems.

This interpretation differs when essentially the most and least secure RGs are chosen. That is the primary report relating to appropriate RGs choice for correct normalization of gene expression in tomato seedlings uncovered to UV-B irradiation.

Simultaneous quantification of the most typical and proteolytic Pseudomonas species in uncooked milk by multiplex qPCR

The warmth-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in uncooked milk, is a significant reason behind destabilization and untimely spoilage of ultra-high temperature (UHT) milk and milk merchandise. To allow fast detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in uncooked milk, we developed two triplex qPCR assays making an allowance for species-dependent variations in AprX exercise. In addition to 5 species-specific hydrolysis probes, focusing on the aprX gene, a common rpoB probe was included within the assay to find out the full Pseudomonas counts.

For all six probes, linear regression strains between Cq worth and goal DNA focus had been obtained in singleplex in addition to in multiplex approaches, yielding R2 values of > 0.975 and amplification efficiencies of 85-97%. Furthermore, excessive specificity was decided utilizing genomic DNA of 75 Pseudomonas strains, assigned to 57 species, and 40 different bacterial species as templates within the qPCR.

Quantification of the goal species and complete Pseudomonas counts resulted in linear detection ranges of approx. 103-107 cfu/ml, which correspond properly to widespread Pseudomonas counts in uncooked milk. Software of the assay utilizing 60 uncooked milk samples from completely different dairies confirmed good settlement of complete Pseudomonas counts calculated by qPCR with cell counts derived from cultivation.

Moreover, a remarkably excessive variability relating to the species composition was noticed for every milk pattern, whereby P. lundensis and P. proteolytica/P. gessardii had been the predominant species detected. KEY POINTS: • Multiplex qPCR for quantification of seven proteolytic Pseudomonas species and complete Pseudomonas counts in uncooked milk • Excessive specificity and sensitivity through hydrolysis probes in opposition to aprX and rpoB • Fast methodology to find out Pseudomonas contamination in uncooked milk and predict spoilage potential.

Selection and optimization of reference genes for RT-qPCR normalization: A case study in Solanum lycopersicum exposed to UV-B

Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Research

Microfluidic platforms provide a drastic enhance in throughput whereas minimizing pattern utilization and hands-on time, which make them essential instruments for large-scale organic research. A spread of such programs have been developed for enzyme exercise research, though their complexity largely hinders their utility to the broader scientific neighborhood.

Right here, we current adaptation of an easy-to-use business microfluidic qPCR system for performing enzyme kinetic research. We exhibit the performance of the Fluidigm Biomark HD system (the Fluidigm system) by figuring out the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The outcomes obtained within the microfluidic system proved reproducible and corresponding to those obtained in a typical microplate-based assay. With a variety of easy-to-use, off-the-shelf parts, the microfluidic system presents itself as a easy and customizable platform for high-throughput enzyme exercise research.

A novel TaqMan qPCR assay for fast detection and quantification of pro-inflammatory microalgae Prototheca spp. in milk samples

Animal or human protothecosis belongs to reasonably uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this examine, we current a multiplex real-time PCR (qPCR) system for fast and actual Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity had been decided.

For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) had been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca.

The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis.

Methylamp One-Step DNA Modification Kit 

P-1010
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  • Ask for price
  • Ask for price
  • 80 Samples
  • 40 Samples
  • 80 Samples

Methylamp Whole Cell Bisulfite Modification Kit 

P-1016
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  • Ask for price
  • 80 Samples
  • 40 Samples
  • 80 Samples

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

Methylamp Global DNA Methylation Quantification Ultra Kit 

P-1014B
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  • 96 Assays
  • 48 Assays
  • 96 Assays

Triplex ASFV Fast qPCR Kit

E0472-01 24reactions Ask for price
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, fast PCR

Triplex ASFV Fast qPCR Kit

E0472-02 96reactions Ask for price
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, fast PCR

Fast SG qPCR Master Mix (2x)

E0411-01 100reactions
EUR 57.77
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR

Fast SG qPCR Master Mix (2x)

E0411-02 200reactions
EUR 107.91
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR

Fast SG qPCR Master Mix (2x)

E0411-03 1000reactions
EUR 510.12
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR

Fast Probe qPCR Master Mix (2x)

E0422-01 100reactions
EUR 55.59
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Fast Probe qPCR Master Mix (2x)

E0422-02 200reactions
EUR 106.82
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Fast Probe qPCR Master Mix (2x)

E0422-03 1000reactions
EUR 467.61
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

1ml Precision FAST qPCR Master Mix

Z-PFAST-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix

Z-PFAST-2ML 2ml
EUR 161
Description: Precision®FAST

Fast SG/ROX qPCR Master Mix (2x)

E0413-01 100reactions
EUR 61.04
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Fast SG/ROX qPCR Master Mix (2x)

E0413-02 200reactions
EUR 116.63
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Fast SG/ROX qPCR Master Mix (2x)

E0413-03 1000reactions
EUR 534.1
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL
EUR 76.3

MethyLight qPCR Probe Kit

RK21241 100RXN
EUR 209.83

2X Universal SYBR Green Fast qPCR Mix  

MBS9141672-25mL 25mL
EUR 755

2X Universal SYBR Green Fast qPCR Mix  

MBS9141672-5mL 5mL
EUR 255

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL
EUR 21.26

1ml Precision FAST CL qPCR Master Mix

Z-PFAST-CL-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST CL qPCR Master Mix

Z-PFAST-CL-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST LR qPCR Master Mix

Z-PFAST-LR-1ML 1ml
EUR 90
Description: Precision®FAST

Fast EvaGreen® Master Mix for qPCR (5000 rxn)

31003-2 50x1ML
EUR 3452
Description: Recombinant Taq polymerase from E. coli

Fast EvaGreen® Master Mix for qPCR (5000 rxn)

31003-2-1 PK
EUR 3452

2X SYBR Green Fast qPCR Mix No Rox

RK21200 50mL
EUR 109

Fast EvaGreen® Master Mix for qPCR(200 rxn)

31003-1 5x1ML
EUR 172
Description: Recombinant Taq polymerase from E. coli

Fast EvaGreen® Master Mix for qPCR (500 rxn)

31003-1-1 PK
EUR 379

2 ml Precision FAST LR qPCR Master Mix

Z-PFAST-LR-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST CL & SY qPCR Master Mix

Z-PFAST-CL-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST CL & SY qPCR Master Mix

Z-PFAST-CL-SY-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST iC & SY qPCR Master Mix

Z-PFAST-iC-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST iC & SY qPCR Master Mix

Z-PFAST-iC-SY-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST LR & SY qPCR Master Mix

Z-PFAST-LR-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST LR & SY qPCR Master Mix

Z-PFAST-LR-SY-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST MX & SY qPCR Master Mix

Z-PFAST-MX-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST MX & SY qPCR Master Mix

Z-PFAST-MX-SY-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST R & SY qPCR Master Mix

Z-PFAST-R-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST R & SY qPCR Master Mix

Z-PFAST-R-SY-2ML 2ml
EUR 161
Description: Precision®FAST

HotStart™ 2X FAST Green qPCR Master Mix

K1171-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

1ml Precision FAST qPCR Master Mix with ROX

Z-PFAST-R-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix with ROX

Z-PFAST-R-2ML 2ml
EUR 161
Description: Precision®FAST

2X SYBR Green Fast qPCR Mix with High ROX

RK21201 50mL
EUR 109

2X SYBR Green Fast qPCR Mix with Low ROXII

RK21202 5mL
EUR 1000.08

Genious 2X SYBR Green Fast qPCR Mix (No ROX)

RK21205 1 ml
EUR 324.28

Fast SG qPCR Master Mix (2x), plus ROX Solution

E0412-01 100reactions
EUR 61.04
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

Fast SG qPCR Master Mix (2x), plus ROX Solution

E0412-02 200reactions
EUR 116.63
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

Fast SG qPCR Master Mix (2x), plus ROX Solution

E0412-03 1000reactions
EUR 534.1
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

Fast Plus EvaGreen® qPCR Master Mix (200 rxn)

31020-1 5x1mL
EUR 201
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix (500 rxn)

31020-1-1 PK
EUR 437

Fast Plus EvaGreen® qPCR Master Mix (5000 rxn)

31020-2 50x1mL
EUR 3970
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix (5000 rxn)

31020-2-1 PK
EUR 3970

5ml Precision FAST qPCR Master Mix (5 x 1ml)

Z-PFAST-5ML 5ml
EUR 348
Description: Precision®FAST

10ml Precision FAST qPCR Master Mix (10 x 1ml)

Z-PFAST-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST qPCR Master Mix (20 x 1ml)

Z-PFAST-20ML 20ml
EUR 1120
Description: Precision®FAST

Fast Probe qPCR Master Mix (2x), plus ROX Solution

E0423-01 100reactions
EUR 58.86
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye

Fast Probe qPCR Master Mix (2x), plus ROX Solution

E0423-02 200reactions
EUR 115.54
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye

Fast Probe qPCR Master Mix (2x), plus ROX Solution

E0423-03 1000reactions
EUR 491.59
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

5ml Precision FAST CL qPCR Master Mix (5 x 1ml)

Z-PFAST-CL-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST iC qPCR Master Mix (5 x 1ml)

Z-PFAST-iC-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST LR qPCR Master Mix (5 x 1ml)

Z-PFAST-LR-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST MX qPCR Master Mix (5 x 1ml)

Z-PFAST-MX-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST SY qPCR Master Mix (5 x 1ml)

Z-PFAST-SY-5ML 5ml
EUR 348
Description: Precision®FAST

1ml Precision FAST qPCR Master Mix for BioRad iCycler

Z-PFAST-iC-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix for BioRad iCycler

Z-PFAST-iC-2ML 2ml
EUR 161
Description: Precision®FAST

10ml Precision FAST CL qPCR Master Mix (10 x 1ml)

Z-PFAST-CL-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST CL qPCR Master Mix(20 x 1ml)

Z-PFAST-CL-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST iC qPCR Master Mix (10 x 1ml)

Z-PFAST-iC-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST iC qPCR Master Mix (20 x 1ml)

Z-PFAST-iC-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST LR qPCR Master Mix (10 x 1ml)

Z-PFAST-LR-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST LR qPCR Master Mix (20 x 1ml)

Z-PFAST-LR-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST MX qPCR Master Mix (10 x 1ml)

Z-PFAST-MX-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST MX qPCR Master Mix (20 x 1ml)

Z-PFAST-MX-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST SY qPCR Master Mix (10 x 1ml)

Z-PFAST-SY-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST SY qPCR Master Mix (20 x 1ml)

Z-PFAST-SY-20ML 20ml
EUR 1120
Description: Precision®FAST

MethyLight qPCR Probe Set

RK21242 100RXN
EUR 209.83

1ml Precision FAST qPCR Master Mix for Stratagene hardware

Z-PFAST-MX-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix for Stratagene hardware

Z-PFAST-MX-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-2ML 2ml
EUR 161
Description: Precision®FAST

Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)

RK21206 1 ml
EUR 76.3

Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed)

RK21207 1 ml
EUR 21.26

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288 1 mL
EUR 101
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17289-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

5ml Precision FAST CL & SY qPCR Master Mix (5 x 1ml)

Z-PFAST-CL-SY-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST iC & SY qPCR Master Mix (5 x 1ml)

Z-PFAST-iC-SY-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST LR & SY qPCR Master Mix (5 x 1ml)

Z-PFAST-LR-SY-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST MX & SY qPCR Master Mix (5 x 1ml)

Z-PFAST-MX-SY-5ML 5ml
EUR 348
Description: Precision®FAST

5ml Precision FAST R & SY qPCR Master Mix (5 x 1ml)

Z-PFAST-R-SY-5ML 5ml
EUR 348
Description: Precision®FAST

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290 1 mL
EUR 101
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291 5 mL
EUR 315
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

10ml Precision FAST CL & SY qPCR Master Mix (10 x 1ml)

Z-PFAST-CL-SY-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST CL & SY qPCR Master Mix (20 x 1ml)

Z-PFAST-CL-SY-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST iC & SY qPCR Master Mix (10 x 1ml)

Z-PFAST-iC-SY-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST iC & SY qPCR Master Mix (20 x 1ml)

Z-PFAST-iC-SY-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST LR & SY qPCR Master Mix (10 x 1ml)

Z-PFAST-LR-SY-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST LR & SY qPCR Master Mix (20 x 1ml)

Z-PFAST-LR-SY-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST MX & SY qPCR Master Mix (10 x 1ml)

Z-PFAST-MX-SY-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST MX & SY qPCR Master Mix (20 x 1ml)

Z-PFAST-MX-SY-20ML 20ml
EUR 1120
Description: Precision®FAST

10ml Precision FAST R & SY qPCR Master Mix (10 x 1ml)

Z-PFAST-R-SY-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST R & SY qPCR Master Mix (20 x 1ml)

Z-PFAST-R-SY-20ML 20ml
EUR 1120
Description: Precision®FAST

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292 1 mL
EUR 101
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293 5 mL
EUR 315
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

5ml Precision FAST qPCR Master Mix with ROX (5 x 1ml)

Z-PFAST-R-5ML 5ml
EUR 348
Description: Precision®FAST

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Fast EvaGreen® Master Mix for qPCR, trial size (100 rxn)

31003-T 1ML
EUR 82
Description: Recombinant Taq polymerase from E. coli

Fast EvaGreen® Master Mix for qPCR, trial size (100 rxn)

31003-T-1 EA
EUR 82

10ml Precision FAST qPCR Master Mix with ROX (10 x 1ml)

Z-PFAST-R-10ML 10ml
EUR 641
Description: Precision®FAST

20ml Precision FAST qPCR Master Mix with ROX (20 x 1ml)

Z-PFAST-R-20ML 20ml
EUR 1120
Description: Precision®FAST

Fast Plus EvaGreen® qPCR Master Mix (trial size, 100 rxn)

31020-T 1mL
EUR 94
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix (trial size, 100 rxn)

31020-T-1 EA
EUR 94

Fast Plus EvaGreen® qPCR Master Mix with low Rox (500 rxn)

31014-1-1 PK
EUR 437

Fast Plus EvaGreen® qPCR Master Mix with low Rox (5000 rxn)

31014-2 50x1mL
EUR 3970
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix with low Rox (5000 rxn)

31014-2-1 PK
EUR 3970

Fast Plus EvaGreen® qPCR Master Mix with high Rox (500 rxn)

31015-1-1 PK
EUR 437

Fast Plus EvaGreen® qPCR Master Mix with high Rox (5000 rxn)

31015-2 50x1mL
EUR 3970
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix with high Rox (5000 rxn)

31015-2-1 PK
EUR 3970

MethyLight 2X qPCR Probe Master Mix

RK21240 1mL
EUR 209.83

7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67020 1 Set
EUR 989
Description: 7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC).

7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67020-1Set 1 Set
EUR 969
Description: 7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC).

VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67002 1 Plate
EUR 99
Description: VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67002-1Plate 1 Plate
EUR 97
Description: VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67004 1 Plate
EUR 99
Description: ROX Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67004-1Plate 1 Plate
EUR 97
Description: ROX Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67006 1 Plate
EUR 99
Description: FAM Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67006-1Plate 1 Plate
EUR 97
Description: FAM Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67010 1 Plate
EUR 99
Description: NED Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67010-1Plate 1 Plate
EUR 97
Description: NED Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67012-1Plate 1 Plate
EUR 97
Description: JOE Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67014 1 Plate
EUR 99
Description: Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block.

Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67014-1Plate 1 Plate
EUR 97
Description: Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block.

Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67016 1Plate
EUR 99
Description: A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments.

SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67008 1 Plate
EUR 99
Description: SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67008-1Plate 1 Plate
EUR 97
Description: SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67000 1 Plate
EUR 99
Description: TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block.

TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

67000-1Plate 1 Plate
EUR 97
Description: TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block.

Fast Plus EvaGreen® Master Mix for qPCR with low Rox (200 rxn)

31014-1 5x1mL
EUR 201
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® Master Mix for qPCR with high Rox (200 rxn)

31015-1 5x1mL
EUR 201
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast-Eva-QPCR-SuperMix-Kit

K5052002 200 rxn
EUR 147.7

Fast Plus EvaGreen® qPCR Master Mix with low Rox (trial size, 100 rxn)

31014-T 1mL
EUR 94
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix with low Rox (trial size, 100 rxn)

31014-T-1 EA
EUR 94

Fast Plus EvaGreen® qPCR Master Mix with High Rox (trial size, 100 rxn)

31015-T 1mL
EUR 94
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Plus EvaGreen® qPCR Master Mix with High Rox (trial size, 100 rxn)

31015-T-1 EA
EUR 94

QPCR Kit DNA Methicillin

MOL8232 EACH
EUR 772.92

QPCR Kit DNA Methicillin

MOL8234 EACH
EUR 982.68

QPCR Kit DNA Methicillin

MOL8236 EACH
EUR 772.92

QPCR Kit DNA Methicillin

MOL8238 EACH
EUR 982.68

rac 4-Methylamphetamine Hydrochloride

M287400 100mg
EUR 1200
Description: 41632-56-8

QPCR Kit DNA Methicillin - EACH

MOL8228 EACH
EUR 1176.51

QPCR Kit QPCR Mastermix DNA - EACH

MOL6354 EACH
EUR 313.2

QPCR Kit DNA Methicillin Mmix - EACH

MOL8230 EACH
EUR 1803.69

Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed

K5058200 200 reactions
EUR 147.7

Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed

K5058400 400 reactions
EUR 247.8

STEC qPCR Kit

TBS42027 100 tests
EUR 198

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)

Q231-EN01 200 rxns
EUR 231.89

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)

Q231-EN01-200rxns 200 rxns
EUR 240.12

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)

Q231-EN02 1,000 rxns
EUR 797.13

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)

Q231-EN02-1000rxns 1,000 rxns
EUR 825.42

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)

Q231-EN03 10,000 rxns
EUR 7464

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)

Q231-EN03-10000rxns 10,000 rxns
EUR 7728.97

qPCR PreMix Kit

MB1001-2 1 Kit
EUR 276
Description: qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers.

Salmonella qPCR Kit

TBS42028 100 tests
EUR 198

QPCR Kit RNA Human Metap - EACH

MOL8024 EACH
EUR 1176.51

ASFV qPCR kit-0517

ZP01340 50T
EUR 150
Description: Blood, tissue, feed and environmental swabs

qPCR AAV Titer Kit

G931 100 rxn
EUR 195
Description: abm’s qPCR AAV Titer Kit is a one-step assay by qPCR which does not require additional lysis, and can be completed within 2 hours. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.

qPCR AAV Titer Kit

E36G931 100 rxn
EUR 271.43

QPCR Kit RNA H10N8

MOL8488 EACH
EUR 788.88

qPCR AAV Titer Kit

MBS8580091-100Reactions 100Reactions
EUR 365

qPCR AAV Titer Kit

MBS8580091-5x100Reactions 5x100Reactions
EUR 1660

Duplex ASFV qPCR Kit

E0471-01 24reactions Ask for price
Description: Certified for veterinary useFor African Swine Fever Virus detection, one internal control, standard PCR

Duplex ASFV qPCR Kit

E0471-02 96reactions Ask for price
Description: Certified for veterinary useFor African Swine Fever Virus detection, one internal control, standard PCR

Triplex ASFV qPCR Kit

E0470-01 24reactions Ask for price
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, standard PCR

Triplex ASFV qPCR Kit

E0470-02 96reactions Ask for price
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, standard PCR

QPCR Kit RNA H10N8Mmix

MOL8490 EACH
EUR 1027.14

Entrans qPCR Probe Kit

RK21209 40 RXN
EUR 185.3

Duplex Babesia qPCR Kit

E0453-01 50reactions
EUR 212.55
Description: For protozoan parasite Babesia spp. detection in animal tissue samples

Duplex Babesia qPCR Kit

E0453-02 100reactions
EUR 392.4
Description: For protozoan parasite Babesia spp. detection in animal tissue samples

ASFV qPCR Detection Kit

E0457-01 24reactions
EUR 107.91
Description: Certified for veterinary useFor African Swine Fever Virus detection

Our work thus provides the likelihood to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby aiding ample corrective measures to be taken.

 

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