Quantitative RT- PCR is without doubt one of the most typical strategies to check gene expression in response to emphasize. Subsequently, it’s essential to have appropriate reference genes (RGs) for outcome normalization. Though a number of experiences describe UV-B-modulated gene expression in Solanum lycopersicum, there aren’t any appropriate RGs recognized till now.
The goal of this work was to guage the suitability of seven conventional genes: actin (ACT), tubulin (TUB), ubiquitin (UBI), glyceraldehyde- Three phosphate dehydrogenase (GAPDH), elongation issue 1α (EF1α), phosphatase 2A catalytic subunit (PP2A) and GAGA binding transcriptional activator (GAGA); and two non-traditional genes: thioredoxin h1 (TRX h1) and UV-B RESISTANCE LOCUS 8 (UVR8), as candidate RGs for his or her potential use as dependable inner controls in leaves, stems and roots of tomato seedlings uncovered to acute and persistent UV-B.
The steadiness of those genes expression was evaluated utilizing 5 statistical algorithms: geNorm, NormFinder, BestKeeper, Delta Ct and ANOVA. Contemplating the excellent stability rating, we suggest ACT+TUB as the perfect pair of RGs for leaves, PP2A+GAPDH+TRX h1 for stems and TUB+UVR8 for roots. The reliability of the chosen RGs for every tissue was verified amplifying tomato chalcone synthase 1 (CHS1) and cyclobutane pyrimidine dimer (CPD) photolyase (PHR1-LIKE). Below UV-B therapy, CHS1 was upregulated in leaves, stems and roots whereas PHR1-LIKE was solely upregulated in leaves and stems.
This interpretation differs when essentially the most and least secure RGs are chosen. That is the primary report relating to appropriate RGs choice for correct normalization of gene expression in tomato seedlings uncovered to UV-B irradiation.
Simultaneous quantification of the most typical and proteolytic Pseudomonas species in uncooked milk by multiplex qPCR
The warmth-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in uncooked milk, is a significant reason behind destabilization and untimely spoilage of ultra-high temperature (UHT) milk and milk merchandise. To allow fast detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in uncooked milk, we developed two triplex qPCR assays making an allowance for species-dependent variations in AprX exercise. In addition to 5 species-specific hydrolysis probes, focusing on the aprX gene, a common rpoB probe was included within the assay to find out the full Pseudomonas counts.
For all six probes, linear regression strains between Cq worth and goal DNA focus had been obtained in singleplex in addition to in multiplex approaches, yielding R2 values of > 0.975 and amplification efficiencies of 85-97%. Furthermore, excessive specificity was decided utilizing genomic DNA of 75 Pseudomonas strains, assigned to 57 species, and 40 different bacterial species as templates within the qPCR.
Quantification of the goal species and complete Pseudomonas counts resulted in linear detection ranges of approx. 103-107 cfu/ml, which correspond properly to widespread Pseudomonas counts in uncooked milk. Software of the assay utilizing 60 uncooked milk samples from completely different dairies confirmed good settlement of complete Pseudomonas counts calculated by qPCR with cell counts derived from cultivation.
Moreover, a remarkably excessive variability relating to the species composition was noticed for every milk pattern, whereby P. lundensis and P. proteolytica/P. gessardii had been the predominant species detected. KEY POINTS: • Multiplex qPCR for quantification of seven proteolytic Pseudomonas species and complete Pseudomonas counts in uncooked milk • Excessive specificity and sensitivity through hydrolysis probes in opposition to aprX and rpoB • Fast methodology to find out Pseudomonas contamination in uncooked milk and predict spoilage potential.
Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Research
Microfluidic platforms provide a drastic enhance in throughput whereas minimizing pattern utilization and hands-on time, which make them essential instruments for large-scale organic research. A spread of such programs have been developed for enzyme exercise research, though their complexity largely hinders their utility to the broader scientific neighborhood.
Right here, we current adaptation of an easy-to-use business microfluidic qPCR system for performing enzyme kinetic research. We exhibit the performance of the Fluidigm Biomark HD system (the Fluidigm system) by figuring out the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The outcomes obtained within the microfluidic system proved reproducible and corresponding to those obtained in a typical microplate-based assay. With a variety of easy-to-use, off-the-shelf parts, the microfluidic system presents itself as a easy and customizable platform for high-throughput enzyme exercise research.
A novel TaqMan qPCR assay for fast detection and quantification of pro-inflammatory microalgae Prototheca spp. in milk samples
Animal or human protothecosis belongs to reasonably uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this examine, we current a multiplex real-time PCR (qPCR) system for fast and actual Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity had been decided.
For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) had been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca.
The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis.
Methylamp One-Step DNA Modification Kit |
P-1010 |
EpiGentek |
-
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-
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-
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|
- 80 Samples
- 40 Samples
- 80 Samples
|
Methylamp Whole Cell Bisulfite Modification Kit |
P-1016 |
EpiGentek |
-
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-
Ask for price
-
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|
- 80 Samples
- 40 Samples
- 80 Samples
|
Methylamp Coupled DNA Isolation & Modification Kit |
P-1002 |
EpiGentek |
40 Samples |
EUR 474.12 |
Methylamp Global DNA Methylation Quantification Ultra Kit |
P-1014B |
EpiGentek |
-
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-
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-
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|
- 96 Assays
- 48 Assays
- 96 Assays
|
Triplex ASFV Fast qPCR Kit |
E0472-01 |
EURx |
24reactions |
Ask for price |
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, fast PCR |
Triplex ASFV Fast qPCR Kit |
E0472-02 |
EURx |
96reactions |
Ask for price |
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, fast PCR |
Fast SG qPCR Master Mix (2x) |
E0411-01 |
EURx |
100reactions |
EUR 57.77 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR |
Fast SG qPCR Master Mix (2x) |
E0411-02 |
EURx |
200reactions |
EUR 107.91 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR |
Fast SG qPCR Master Mix (2x) |
E0411-03 |
EURx |
1000reactions |
EUR 510.12 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR |
Fast Probe qPCR Master Mix (2x) |
E0422-01 |
EURx |
100reactions |
EUR 55.59 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes |
Fast Probe qPCR Master Mix (2x) |
E0422-02 |
EURx |
200reactions |
EUR 106.82 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes |
Fast Probe qPCR Master Mix (2x) |
E0422-03 |
EURx |
1000reactions |
EUR 467.61 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes |
1ml Precision FAST qPCR Master Mix |
Z-PFAST-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix |
Z-PFAST-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
Fast SG/ROX qPCR Master Mix (2x) |
E0413-01 |
EURx |
100reactions |
EUR 61.04 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
Fast SG/ROX qPCR Master Mix (2x) |
E0413-02 |
EURx |
200reactions |
EUR 116.63 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
Fast SG/ROX qPCR Master Mix (2x) |
E0413-03 |
EURx |
1000reactions |
EUR 534.1 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye |
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
EUR 76.3 |
|
MethyLight qPCR Probe Kit |
RK21241 |
Abclonal |
100RXN |
EUR 209.83 |
2X Universal SYBR Green Fast qPCR Mix |
MBS9141672-25mL |
MyBiosource |
25mL |
EUR 755 |
2X Universal SYBR Green Fast qPCR Mix |
MBS9141672-5mL |
MyBiosource |
5mL |
EUR 255 |
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
EUR 21.26 |
|
1ml Precision FAST CL qPCR Master Mix |
Z-PFAST-CL-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST CL qPCR Master Mix |
Z-PFAST-CL-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST LR qPCR Master Mix |
Z-PFAST-LR-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
Fast EvaGreen® Master Mix for qPCR (5000 rxn) |
31003-2 |
Biotium |
50x1ML |
EUR 3452 |
|
Description: Recombinant Taq polymerase from E. coli |
Fast EvaGreen® Master Mix for qPCR (5000 rxn) |
31003-2-1 |
Biotium |
PK |
EUR 3452 |
2X SYBR Green Fast qPCR Mix No Rox |
RK21200 |
Abclonal |
50mL |
EUR 109 |
Fast EvaGreen® Master Mix for qPCR(200 rxn) |
31003-1 |
Biotium |
5x1ML |
EUR 172 |
|
Description: Recombinant Taq polymerase from E. coli |
Fast EvaGreen® Master Mix for qPCR (500 rxn) |
31003-1-1 |
Biotium |
PK |
EUR 379 |
2 ml Precision FAST LR qPCR Master Mix |
Z-PFAST-LR-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST CL & SY qPCR Master Mix |
Z-PFAST-CL-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST CL & SY qPCR Master Mix |
Z-PFAST-CL-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST iC & SY qPCR Master Mix |
Z-PFAST-iC-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST iC & SY qPCR Master Mix |
Z-PFAST-iC-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST LR & SY qPCR Master Mix |
Z-PFAST-LR-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST LR & SY qPCR Master Mix |
Z-PFAST-LR-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST MX & SY qPCR Master Mix |
Z-PFAST-MX-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST MX & SY qPCR Master Mix |
Z-PFAST-MX-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST R & SY qPCR Master Mix |
Z-PFAST-R-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST R & SY qPCR Master Mix |
Z-PFAST-R-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
HotStart™ 2X FAST Green qPCR Master Mix |
K1171-25 |
ApexBio |
25ml |
EUR 424 |
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
K1171-5 |
ApexBio |
5ml |
EUR 132 |
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
K1171-50 |
ApexBio |
50ml |
EUR 826.4 |
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
1ml Precision FAST qPCR Master Mix with ROX |
Z-PFAST-R-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix with ROX |
Z-PFAST-R-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
2X SYBR Green Fast qPCR Mix with High ROX |
RK21201 |
Abclonal |
50mL |
EUR 109 |
2X SYBR Green Fast qPCR Mix with Low ROXII |
RK21202 |
Abclonal |
5mL |
EUR 1000.08 |
Genious 2X SYBR Green Fast qPCR Mix (No ROX) |
RK21205 |
Abclonal |
1 ml |
EUR 324.28 |
|
Fast SG qPCR Master Mix (2x), plus ROX Solution |
E0412-01 |
EURx |
100reactions |
EUR 61.04 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye |
Fast SG qPCR Master Mix (2x), plus ROX Solution |
E0412-02 |
EURx |
200reactions |
EUR 116.63 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye |
Fast SG qPCR Master Mix (2x), plus ROX Solution |
E0412-03 |
EURx |
1000reactions |
EUR 534.1 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye |
Fast Plus EvaGreen® qPCR Master Mix (200 rxn) |
31020-1 |
Biotium |
5x1mL |
EUR 201 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix (500 rxn) |
31020-1-1 |
Biotium |
PK |
EUR 437 |
Fast Plus EvaGreen® qPCR Master Mix (5000 rxn) |
31020-2 |
Biotium |
50x1mL |
EUR 3970 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix (5000 rxn) |
31020-2-1 |
Biotium |
PK |
EUR 3970 |
5ml Precision FAST qPCR Master Mix (5 x 1ml) |
Z-PFAST-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
10ml Precision FAST qPCR Master Mix (10 x 1ml) |
Z-PFAST-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST qPCR Master Mix (20 x 1ml) |
Z-PFAST-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
Fast Probe qPCR Master Mix (2x), plus ROX Solution |
E0423-01 |
EURx |
100reactions |
EUR 58.86 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye |
Fast Probe qPCR Master Mix (2x), plus ROX Solution |
E0423-02 |
EURx |
200reactions |
EUR 115.54 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye |
Fast Probe qPCR Master Mix (2x), plus ROX Solution |
E0423-03 |
EURx |
1000reactions |
EUR 491.59 |
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
K1172-25 |
ApexBio |
25ml |
EUR 424 |
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
K1172-5 |
ApexBio |
5ml |
EUR 132 |
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
K1172-50 |
ApexBio |
50ml |
EUR 826.4 |
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
5ml Precision FAST CL qPCR Master Mix (5 x 1ml) |
Z-PFAST-CL-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST iC qPCR Master Mix (5 x 1ml) |
Z-PFAST-iC-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST LR qPCR Master Mix (5 x 1ml) |
Z-PFAST-LR-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST MX qPCR Master Mix (5 x 1ml) |
Z-PFAST-MX-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST SY qPCR Master Mix (5 x 1ml) |
Z-PFAST-SY-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
1ml Precision FAST qPCR Master Mix for BioRad iCycler |
Z-PFAST-iC-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix for BioRad iCycler |
Z-PFAST-iC-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
10ml Precision FAST CL qPCR Master Mix (10 x 1ml) |
Z-PFAST-CL-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST CL qPCR Master Mix(20 x 1ml) |
Z-PFAST-CL-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST iC qPCR Master Mix (10 x 1ml) |
Z-PFAST-iC-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST iC qPCR Master Mix (20 x 1ml) |
Z-PFAST-iC-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST LR qPCR Master Mix (10 x 1ml) |
Z-PFAST-LR-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST LR qPCR Master Mix (20 x 1ml) |
Z-PFAST-LR-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST MX qPCR Master Mix (10 x 1ml) |
Z-PFAST-MX-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST MX qPCR Master Mix (20 x 1ml) |
Z-PFAST-MX-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST SY qPCR Master Mix (10 x 1ml) |
Z-PFAST-SY-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST SY qPCR Master Mix (20 x 1ml) |
Z-PFAST-SY-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
MethyLight qPCR Probe Set |
RK21242 |
Abclonal |
100RXN |
EUR 209.83 |
1ml Precision FAST qPCR Master Mix for Stratagene hardware |
Z-PFAST-MX-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix for Stratagene hardware |
Z-PFAST-MX-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
1ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
Z-PFAST-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
Z-PFAST-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed) |
RK21206 |
Abclonal |
1 ml |
EUR 76.3 |
|
Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed) |
RK21207 |
Abclonal |
1 ml |
EUR 21.26 |
|
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288 |
AAT Bioquest |
1 mL |
EUR 101 |
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
5ml Precision FAST CL & SY qPCR Master Mix (5 x 1ml) |
Z-PFAST-CL-SY-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST iC & SY qPCR Master Mix (5 x 1ml) |
Z-PFAST-iC-SY-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST LR & SY qPCR Master Mix (5 x 1ml) |
Z-PFAST-LR-SY-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST MX & SY qPCR Master Mix (5 x 1ml) |
Z-PFAST-MX-SY-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
5ml Precision FAST R & SY qPCR Master Mix (5 x 1ml) |
Z-PFAST-R-SY-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290 |
AAT Bioquest |
1 mL |
EUR 101 |
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291 |
AAT Bioquest |
5 mL |
EUR 315 |
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
10ml Precision FAST CL & SY qPCR Master Mix (10 x 1ml) |
Z-PFAST-CL-SY-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST CL & SY qPCR Master Mix (20 x 1ml) |
Z-PFAST-CL-SY-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST iC & SY qPCR Master Mix (10 x 1ml) |
Z-PFAST-iC-SY-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST iC & SY qPCR Master Mix (20 x 1ml) |
Z-PFAST-iC-SY-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST LR & SY qPCR Master Mix (10 x 1ml) |
Z-PFAST-LR-SY-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST LR & SY qPCR Master Mix (20 x 1ml) |
Z-PFAST-LR-SY-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST MX & SY qPCR Master Mix (10 x 1ml) |
Z-PFAST-MX-SY-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST MX & SY qPCR Master Mix (20 x 1ml) |
Z-PFAST-MX-SY-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
10ml Precision FAST R & SY qPCR Master Mix (10 x 1ml) |
Z-PFAST-R-SY-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST R & SY qPCR Master Mix (20 x 1ml) |
Z-PFAST-R-SY-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292 |
AAT Bioquest |
1 mL |
EUR 101 |
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293 |
AAT Bioquest |
5 mL |
EUR 315 |
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
5ml Precision FAST qPCR Master Mix with ROX (5 x 1ml) |
Z-PFAST-R-5ML |
Novacyt Group |
5ml |
EUR 348 |
Description: Precision®FAST |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
Fast EvaGreen® Master Mix for qPCR, trial size (100 rxn) |
31003-T |
Biotium |
1ML |
EUR 82 |
|
Description: Recombinant Taq polymerase from E. coli |
Fast EvaGreen® Master Mix for qPCR, trial size (100 rxn) |
31003-T-1 |
Biotium |
EA |
EUR 82 |
10ml Precision FAST qPCR Master Mix with ROX (10 x 1ml) |
Z-PFAST-R-10ML |
Novacyt Group |
10ml |
EUR 641 |
Description: Precision®FAST |
20ml Precision FAST qPCR Master Mix with ROX (20 x 1ml) |
Z-PFAST-R-20ML |
Novacyt Group |
20ml |
EUR 1120 |
Description: Precision®FAST |
Fast Plus EvaGreen® qPCR Master Mix (trial size, 100 rxn) |
31020-T |
Biotium |
1mL |
EUR 94 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix (trial size, 100 rxn) |
31020-T-1 |
Biotium |
EA |
EUR 94 |
Fast Plus EvaGreen® qPCR Master Mix with low Rox (500 rxn) |
31014-1-1 |
Biotium |
PK |
EUR 437 |
Fast Plus EvaGreen® qPCR Master Mix with low Rox (5000 rxn) |
31014-2 |
Biotium |
50x1mL |
EUR 3970 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix with low Rox (5000 rxn) |
31014-2-1 |
Biotium |
PK |
EUR 3970 |
Fast Plus EvaGreen® qPCR Master Mix with high Rox (500 rxn) |
31015-1-1 |
Biotium |
PK |
EUR 437 |
Fast Plus EvaGreen® qPCR Master Mix with high Rox (5000 rxn) |
31015-2 |
Biotium |
50x1mL |
EUR 3970 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix with high Rox (5000 rxn) |
31015-2-1 |
Biotium |
PK |
EUR 3970 |
MethyLight 2X qPCR Probe Master Mix |
RK21240 |
Abclonal |
1mL |
EUR 209.83 |
7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67020 |
AAT Bioquest |
1 Set |
EUR 989 |
Description: 7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC). |
7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67020-1Set |
AAT Bioquest |
1 Set |
EUR 969 |
|
Description: 7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC). |
VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67002 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67002-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67004 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: ROX Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67004-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: ROX Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67006 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: FAM Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67006-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: FAM Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67010 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: NED Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67010-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: NED Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67012-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: JOE Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67014 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block. |
Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67014-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block. |
Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67016 |
AAT Bioquest |
1Plate |
EUR 99 |
Description: A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments. |
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67008 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67008-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. |
TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67000 |
AAT Bioquest |
1 Plate |
EUR 99 |
Description: TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block. |
TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
67000-1Plate |
AAT Bioquest |
1 Plate |
EUR 97 |
|
Description: TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block. |
Fast Plus EvaGreen® Master Mix for qPCR with low Rox (200 rxn) |
31014-1 |
Biotium |
5x1mL |
EUR 201 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® Master Mix for qPCR with high Rox (200 rxn) |
31015-1 |
Biotium |
5x1mL |
EUR 201 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast-Eva-QPCR-SuperMix-Kit |
K5052002 |
Biochain |
200 rxn |
EUR 147.7 |
Fast Plus EvaGreen® qPCR Master Mix with low Rox (trial size, 100 rxn) |
31014-T |
Biotium |
1mL |
EUR 94 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix with low Rox (trial size, 100 rxn) |
31014-T-1 |
Biotium |
EA |
EUR 94 |
Fast Plus EvaGreen® qPCR Master Mix with High Rox (trial size, 100 rxn) |
31015-T |
Biotium |
1mL |
EUR 94 |
|
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli |
Fast Plus EvaGreen® qPCR Master Mix with High Rox (trial size, 100 rxn) |
31015-T-1 |
Biotium |
EA |
EUR 94 |
Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed |
K5058200 |
Biochain |
200 reactions |
EUR 147.7 |
Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed |
K5058400 |
Biochain |
400 reactions |
EUR 247.8 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
Q231-EN01 |
Vazyme |
200 rxns |
EUR 231.89 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
Q231-EN01-200rxns |
Vazyme |
200 rxns |
EUR 240.12 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
Q231-EN02 |
Vazyme |
1,000 rxns |
EUR 797.13 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
Q231-EN02-1000rxns |
Vazyme |
1,000 rxns |
EUR 825.42 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
Q231-EN03 |
Vazyme |
10,000 rxns |
EUR 7464 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
Q231-EN03-10000rxns |
Vazyme |
10,000 rxns |
EUR 7728.97 |
qPCR PreMix Kit |
MB1001-2 |
BosterBio |
1 Kit |
EUR 276 |
Description: qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers. |
ASFV qPCR kit-0517 |
ZP01340 |
Ring Biotechnology Co |
50T |
EUR 150 |
Description: Blood, tissue, feed and environmental swabs |
qPCR AAV Titer Kit |
G931 |
ABM |
100 rxn |
EUR 195 |
Description: abm’s qPCR AAV Titer Kit is a one-step assay by qPCR which does not require additional lysis, and can be completed within 2 hours. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. |
qPCR AAV Titer Kit |
E36G931 |
EnoGene |
100 rxn |
EUR 271.43 |
qPCR AAV Titer Kit |
MBS8580091-100Reactions |
MyBiosource |
100Reactions |
EUR 365 |
qPCR AAV Titer Kit |
MBS8580091-5x100Reactions |
MyBiosource |
5x100Reactions |
EUR 1660 |
Duplex ASFV qPCR Kit |
E0471-01 |
EURx |
24reactions |
Ask for price |
Description: Certified for veterinary useFor African Swine Fever Virus detection, one internal control, standard PCR |
Duplex ASFV qPCR Kit |
E0471-02 |
EURx |
96reactions |
Ask for price |
Description: Certified for veterinary useFor African Swine Fever Virus detection, one internal control, standard PCR |
Triplex ASFV qPCR Kit |
E0470-01 |
EURx |
24reactions |
Ask for price |
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, standard PCR |
Triplex ASFV qPCR Kit |
E0470-02 |
EURx |
96reactions |
Ask for price |
Description: Certified for veterinary useFor African Swine Fever Virus detection, two internal controls, standard PCR |
Entrans qPCR Probe Kit |
RK21209 |
Abclonal |
40 RXN |
EUR 185.3 |
|
Duplex Babesia qPCR Kit |
E0453-01 |
EURx |
50reactions |
EUR 212.55 |
Description: For protozoan parasite Babesia spp. detection in animal tissue samples |
Duplex Babesia qPCR Kit |
E0453-02 |
EURx |
100reactions |
EUR 392.4 |
Description: For protozoan parasite Babesia spp. detection in animal tissue samples |
ASFV qPCR Detection Kit |
E0457-01 |
EURx |
24reactions |
EUR 107.91 |
Description: Certified for veterinary useFor African Swine Fever Virus detection |
Our work thus provides the likelihood to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby aiding ample corrective measures to be taken.
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