Selection and optimization of reference genes for RT-qPCR normalization: A case study in Solanum lycopersicum exposed to UV-B

Quantitative RT- PCR is without doubt one of the most typical strategies to check gene expression in response to emphasize. Subsequently, it’s essential to have appropriate reference genes (RGs) for outcome normalization. Though a number of experiences describe UV-B-modulated gene expression in Solanum lycopersicum, there aren’t any appropriate RGs recognized till now.

The goal of this work was to guage the suitability of seven conventional genes: actin (ACT), tubulin (TUB), ubiquitin (UBI), glyceraldehyde- Three phosphate dehydrogenase (GAPDH), elongation issue 1α (EF1α), phosphatase 2A catalytic subunit (PP2A) and GAGA binding transcriptional activator (GAGA); and two non-traditional genes: thioredoxin h1 (TRX h1) and UV-B RESISTANCE LOCUS 8 (UVR8), as candidate RGs for his or her potential use as dependable inner controls in leaves, stems and roots of tomato seedlings uncovered to acute and persistent UV-B.

The steadiness of those genes expression was evaluated utilizing 5 statistical algorithms: geNorm, NormFinder, BestKeeper, Delta Ct and ANOVA. Contemplating the excellent stability rating, we suggest ACT+TUB as the perfect pair of RGs for leaves, PP2A+GAPDH+TRX h1 for stems and TUB+UVR8 for roots. The reliability of the chosen RGs for every tissue was verified amplifying tomato chalcone synthase 1 (CHS1) and cyclobutane pyrimidine dimer (CPD) photolyase (PHR1-LIKE). Below UV-B therapy, CHS1 was upregulated in leaves, stems and roots whereas PHR1-LIKE was solely upregulated in leaves and stems.

This interpretation differs when essentially the most and least secure RGs are chosen. That is the primary report relating to appropriate RGs choice for correct normalization of gene expression in tomato seedlings uncovered to UV-B irradiation.

Simultaneous quantification of the most typical and proteolytic Pseudomonas species in uncooked milk by multiplex qPCR

The warmth-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in uncooked milk, is a significant reason behind destabilization and untimely spoilage of ultra-high temperature (UHT) milk and milk merchandise. To allow fast detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in uncooked milk, we developed two triplex qPCR assays making an allowance for species-dependent variations in AprX exercise. In addition to 5 species-specific hydrolysis probes, focusing on the aprX gene, a common rpoB probe was included within the assay to find out the full Pseudomonas counts.

For all six probes, linear regression strains between Cq worth and goal DNA focus had been obtained in singleplex in addition to in multiplex approaches, yielding R2 values of > 0.975 and amplification efficiencies of 85-97%. Furthermore, excessive specificity was decided utilizing genomic DNA of 75 Pseudomonas strains, assigned to 57 species, and 40 different bacterial species as templates within the qPCR.

Quantification of the goal species and complete Pseudomonas counts resulted in linear detection ranges of approx. 103-107 cfu/ml, which correspond properly to widespread Pseudomonas counts in uncooked milk. Software of the assay utilizing 60 uncooked milk samples from completely different dairies confirmed good settlement of complete Pseudomonas counts calculated by qPCR with cell counts derived from cultivation.

Moreover, a remarkably excessive variability relating to the species composition was noticed for every milk pattern, whereby P. lundensis and P. proteolytica/P. gessardii had been the predominant species detected. KEY POINTS: • Multiplex qPCR for quantification of seven proteolytic Pseudomonas species and complete Pseudomonas counts in uncooked milk • Excessive specificity and sensitivity through hydrolysis probes in opposition to aprX and rpoB • Fast methodology to find out Pseudomonas contamination in uncooked milk and predict spoilage potential.

Selection and optimization of reference genes for RT-qPCR normalization: A case study in Solanum lycopersicum exposed to UV-B

Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Research

Microfluidic platforms provide a drastic enhance in throughput whereas minimizing pattern utilization and hands-on time, which make them essential instruments for large-scale organic research. A spread of such programs have been developed for enzyme exercise research, though their complexity largely hinders their utility to the broader scientific neighborhood.

Right here, we current adaptation of an easy-to-use business microfluidic qPCR system for performing enzyme kinetic research. We exhibit the performance of the Fluidigm Biomark HD system (the Fluidigm system) by figuring out the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The outcomes obtained within the microfluidic system proved reproducible and corresponding to those obtained in a typical microplate-based assay. With a variety of easy-to-use, off-the-shelf parts, the microfluidic system presents itself as a easy and customizable platform for high-throughput enzyme exercise research.

A novel TaqMan qPCR assay for fast detection and quantification of pro-inflammatory microalgae Prototheca spp. in milk samples

Animal or human protothecosis belongs to reasonably uncommon, endemic, pro-inflammatory infections. It’s brought on by achlorophyllous algae of the genus Prototheca. Particularly, P. bovis (previously P. zopfii genotype 2) is usually inflected as a non-bacterial causative agent of dairy cattle mastitis. On this examine, we current a multiplex real-time PCR (qPCR) system for fast and actual Prototheca spp. detection and quantification. Restrict of detection, diagnostic sensitivity, and specificity had been decided.

For the primary time, particular sequences of AccD (encoding acetyl CoA reductase) for P. bovis, cox1 (encoding cytochrome C oxidase subunit 1) for P. wickerhamii, cytB (encoding cytochrome B) for P. blashkeae and atp6 (encoding transporting ATPase F0 subunit 6) for P. ciferrii (previously P. zopfii genotype 1) had been used for species identification and quantification along with 28S rRNA sequence detecting genus Prototheca.

The developed qPCR assay was utilized to 55 particular person cow milk samples from a herd suspected of protothecosis, 41 bulk milk samples from completely different Czech farms, 16 boxed milk samples bought in supermarkets and 21 environmental samples originating from a farm suspected of protothecosis.

Methylamp One-Step DNA Modification Kit 
P-1010
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
  • 80 Samples
  • 40 Samples
  • 80 Samples

Methylamp Whole Cell Bisulfite Modification Kit 
P-1016
  • EUR 595.92
  • EUR 213.40
  • EUR 380.60
  • 80 Samples
  • 40 Samples
  • 80 Samples

Methylamp Global DNA Methylation Quantification Ultra Kit 
P-1014B
  • EUR 1137.06
  • EUR 429.00
  • EUR 760.10
  • 96 Assays
  • 48 Assays
  • 96 Assays

Methylamp Coupled DNA Isolation & Modification Kit
P-1002 40 Samples
EUR 474.12

Fast Eva QPCR SuperMix Kit
K5052002 200 rxn
EUR 188

Fast Pro QPCR SuperMix Kit - ROX premixed
K5058200 200 reactions
EUR 188

Fast Pro QPCR SuperMix Kit - ROX premixed
K5058400 400 reactions
EUR 316

Fast EvaGreen Master Mix for qPCR (5000 rxn)
31003-2 50x1ML
EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast EvaGreen Master Mix for qPCR(200 rxn)
31003 2x1ML
EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)
31003-1 5x1ML
EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Genious 2X SYBR Green Fast qPCR Mix
RK21204 1 mL Ask for price

2X Universal SYBR Green Fast qPCR Mix
RK21203 1 mL Ask for price

Fast Plus EvaGreen qPCR Master Mix (500 rxn)
31020-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX)
RK21206 1 ml Ask for price

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)
RK21205 1 ml Ask for price

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)
31003-T 1ML
EUR 147.6
Description: Minimum order quantity: 1 unit of 1ML

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)
31020-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17288-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17289-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17290-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17291-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17292-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17293-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)
31014 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)
31014-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
31014-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)
31015-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17288 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17289 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17290 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17291 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17292 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17293 5 mL
EUR 308

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
31015 2x1mL
EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)
31015-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
17276 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
17277 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17278 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17279 5 mL
EUR 308

7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67020 1 Set
EUR 969

7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67020-1Set 1 Set
EUR 969
Description: 7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC).

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
17280 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
17281 5 mL
EUR 308

VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67002 1 Plate
EUR 97

ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67004 1 Plate
EUR 97

FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67006 1 Plate
EUR 97

NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67010 1 Plate
EUR 97

JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67012 1 Plate
EUR 97

Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67014 1 Plate
EUR 97

VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67002-1Plate 1 Plate
EUR 97
Description: VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67004-1Plate 1 Plate
EUR 97
Description: ROX Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67006-1Plate 1 Plate
EUR 97
Description: FAM Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67010-1Plate 1 Plate
EUR 97
Description: NED Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67012-1Plate 1 Plate
EUR 97
Description: JOE Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67014-1Plate 1 Plate
EUR 97
Description: Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block.

SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67008 1 Plate
EUR 97

SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67008-1Plate 1 Plate
EUR 97
Description: SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block.

QPCR Kit DNA Methicillin
MOL8228 EACH
EUR 1045.79

QPCR Kit DNA Methicillin
MOL8232 EACH
EUR 772.92

QPCR Kit DNA Methicillin
MOL8234 EACH
EUR 982.68

QPCR Kit DNA Methicillin
MOL8236 EACH
EUR 772.92

QPCR Kit DNA Methicillin
MOL8238 EACH
EUR 982.68

TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67000 1 Plate
EUR 97

TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
67000-1Plate 1 Plate
EUR 97
Description: TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block.

Methylamine
S-2457 1ML
EUR 44.4

QPCR Kit DNA Methicillin Mmix
MOL8230 EACH
EUR 1603.28

Methylamine HCl
abx187854-100g 100 g
EUR 444

Methyl3-(methylamino)propanoate
20-abx180820
  • EUR 961.20
  • EUR 326.40
  • EUR 1562.40
  • EUR 627.60
  • 10 g
  • 1 g
  • 25 g
  • 5 g

Methyl-PEG3-MS
abx186609-250mg 250 mg
EUR 526.8

3-(Methylamino)-1-Propanol
20-abx183279
  • EUR 526.80
  • EUR 260.40
  • EUR 393.60
  • 10 g
  • 1 g
  • 5 g

2-Methylamino Thiourea
abx187868-100g 100 g
EUR 360

2-(Methylamino)-1H-purin-6(7H)-one
HY-101412 10mg
EUR 456

2-Methylaminoethanol
20-abx183240
  • EUR 360.00
  • EUR 309.60
  • 1 kg
  • 500 g

4-(Methylamino)Pyrimidine
20-abx185165
  • EUR 1312.80
  • EUR 644.40
  • 1 g
  • 250 mg

QPCR Kit QPCR Mastermix DNA
MOL6354 EACH
EUR 278.4

(2-Bromobenzyl)methylamine
20-abx185678
  • EUR 326.40
  • EUR 1362.00
  • EUR 594.00
  • 1 g
  • 25 g
  • 5 g

METHYLAMINE HYDROCHLORIDE
913015 each Ask for price

Methylamine Hydrochloride
S-2457A 1ML
EUR 63.6

4-Methylaminophenol sulfate
GK4728-100G 100 g
EUR 63.6

4-Methylaminophenol sulfate
GK4728-1KG 1 kg
EUR 223.2

4-Methylaminophenol sulfate
GK4728-250G 250 g
EUR 93.6

4-Methylaminophenol sulfate
GK4728-25G 25 g
EUR 49.2

4-Methylaminophenol sulfate
GK4728-500G 500 g
EUR 141.6

4-Methylaminophenol sulfate
GK4728-1 1
EUR 154.3

4-Methylaminophenol sulfate
GK4728-100 100
EUR 22.9

4-Methylaminophenol sulfate
GK4728-25 25
EUR 12.1

4-Methylaminophenol sulfate
GK4728-250 250
EUR 47.4

4-Methylaminophenol sulfate
GK4728-500 500
EUR 87.1

4-[(Methylamino)methyl]-1,2-benzenediol hydrobromide
20-abx180489
  • EUR 360.00
  • EUR 644.40
  • 1 g
  • 5 g

(S)-N-[1-(2-Fluorophenyl)Ethyl]Methylamine
abx188040-25g 25 g
EUR 3717.6

(5-Fluoropyridin-3-yl)methylamine
20-abx181432
  • EUR 2464.80
  • EUR 661.20
  • EUR 376.80
  • EUR 1579.20
  • 10 g
  • 1 g
  • 250 mg
  • 5 g

(1-Benzyl-piperidin-4-yl)-methyl-amine
20-abx185674
  • EUR 477.60
  • EUR 376.80
  • 10 g
  • 5 g

6-(Methylamino)Pyridin-3-Ylboronic Acid
abx186345-1g 1 g
EUR 2431.2

BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole]
23051 5 mg
EUR 86

BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole]
23051-5mg 5 mg
EUR 86
Description: BTA-1 is a fluorescent Thioflavin-T derivative that exhibits high affinity for amyloid deposits (Ki = 11 nM for Ab 40).

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)
Q231-EN01 200 rxns
EUR 185.51

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)
Q231-EN02 1,000 rxns
EUR 637.7

AccurSTART One Step RT-qPCR Probe Kit (FOR FAST)
Q231-EN03 10,000 rxns
EUR 5971.2

1-Methyl-aminomethyl-naphthalene
abx180191-100g 100 g
EUR 510

1-Methyl-5-aminomethylimidazole
abx181581-1g 1 g
EUR 1011.6

(1,3-Dimethyl-1H-pyrazol-4-yl)methylamine
20-abx180081
  • EUR 460.80
  • EUR 961.20
  • 1 g
  • 5 g

Methylase ELISA Kit| Rat Methylase ELISA Kit
EF017882 96 Tests
EUR 826.8

3-(Methylamino)pyridazine-4-carbonitrile
20-abx181853
  • EUR 1528.80
  • EUR 4168.80
  • 1 g
  • 5 g

Rat Methylase(Methylase ) ELISA Kit
ER1147 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.875pg/ml

Methyl2-amino-3-mercaptopropanoate,HCl
abx180819-1g 1 g
EUR 543.6

QPCR Kit RNA Human Metap
MOL8024 EACH
EUR 1045.79

5-Pyrimidinecarboxylic acid, 2-(methylamino)- (7CI,8CI,9CI)
20-abx183759
  • EUR 3032.40
  • EUR 678.00
  • EUR 1863.60
  • 10 g
  • 1 g
  • 5 g

(2,4-Dichlorobenzyl)methylamine hydrochloride
20-abx185677
  • EUR 861.60
  • EUR 226.80
  • EUR 944.40
  • EUR 276.00
  • 100 g
  • 1 g
  • 25 g
  • 5 g

Methyl3-aminopyridazine-4-carboxylate
20-abx185501
  • EUR 343.20
  • EUR 828.00
  • EUR 493.20
  • 100 mg
  • 1 g
  • 250 mg

C-(6-Bromo-Pyridin-3-Yl)-Methylamine Hydrochloride
20-abx182390
  • EUR 1713.60
  • EUR 477.60
  • EUR 1111.20
  • 10 g
  • 1 g
  • 5 g

C-(5-Bromo-Thiophen-2-Yl)-Methylamine Hydrochloride
20-abx186396
  • EUR 444.00
  • EUR 1028.40
  • 1 g
  • 5 g

1-Benzyl-4-(methylamino)-4-piperidinecarbonitrile
abx184928-1g 1 g
EUR 1078.8

(R)-Tert-Butyl 3-(Methylamino)Pyrrolidine-1-Carboxylate
abx188026-250g 250 g
EUR 4419.6

2-Chloro-4-(methylamino)-5-Pyrimidinecarboxaldehyde
20-abx181760
  • EUR 2815.20
  • EUR 1596.00
  • 1 g
  • 250 mg

N-Tris (Hydroxymethyl)Methyl-2-Aminoethane Sulfonic Acid)
42020092-1 100 g
EUR 137.27

N-Tris (Hydroxymethyl)Methyl-2-Aminoethane Sulfonic Acid)
42020092-2 500 g
EUR 549.05

N-Tris (Hydroxymethyl)Methyl-2-Aminoethane Sulfonic Acid)
42020092-3 1 kg
EUR 718.34

QPCR Kit RNA Human Metap Mmix
MOL8026 EACH
EUR 1368.27

JBS Methylation Kit
M-CS-510 6 reactions
EUR 296
Description: JBS Methylation Kit

Rat Methylase ELISA Kit
CN-01810R1 96T
EUR 530.4

Rat Methylase ELISA Kit
CN-01810R2 48T
EUR 351.6

Pig Methylase ELISA kit
E07M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Methylase ELISA kit
E07M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Methylase ELISA kit
E07M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Methylase ELISA kit
E08M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Methylase ELISA kit
E08M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Methylase ELISA kit
E08M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Methylase ELISA Kit
ERM0270 96Tests
EUR 625.2

Rat Methylase ELISA kit
E02M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Methylase ELISA kit
E02M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Methylase ELISA kit
E02M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Methylase ELISA Kit
GA-E0339RT-48T 48T
EUR 380.4

Rat Methylase ELISA Kit
GA-E0339RT-96T 96T
EUR 595.2

Rat Methylase ELISA Kit
QY-E11313 96T
EUR 433.2

Rat Methylase ELISA Kit
YLA0910RA-48T 48T
EUR 465

Rat Methylase ELISA Kit
YLA0910RA-96T 96T
EUR 600

Rat Methylase ELISA Kit
SL0495Ra -
EUR 498

Goat Methylase ELISA kit
E06M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Methylase ELISA kit
E06M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Methylase ELISA kit
E06M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Methylase ELISA Kit
EGTM0270 96Tests
EUR 625.2

QPCR Kit RNA H10N8
MOL8488 EACH
EUR 788.88

qPCR AAV Titer Kit
E36G931 100 rxn
EUR 271.43

Human Methylase ELISA Kit
201-12-0927 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Mouse Methylase ELISA Kit 　
CN-02688M1 96T
EUR 565.2

Mouse Methylase ELISA Kit 　
CN-02688M2 48T
EUR 386.4

Human Methylase ELISA Kit
CN-03550H1 96T
EUR 556.8

Human Methylase ELISA Kit
CN-03550H2 48T
EUR 375.6

Mouse Methylase ELISA Kit
EMM0270 96Tests
EUR 625.2

Mouse Methylase ELISA kit
E03M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Methylase ELISA kit
E03M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Methylase ELISA kit
E03M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Methylase ELISA kit
E01M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Methylase ELISA kit
E01M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Methylase ELISA kit
E01M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Methylase ELISA Kit
GA-E0943HM-48T 48T
EUR 346.8

Human Methylase ELISA Kit
GA-E0943HM-96T 96T
EUR 559.2

Mouse Methylase ELISA Kit 　 　 　
GA-E0324MS-48T 48T
EUR 403.2

Mouse Methylase ELISA Kit 　 　 　
GA-E0324MS-96T 96T
EUR 640.8

Human Methylase ELISA Kit
EHM0270 96Tests
EUR 625.2

Mouse Methylase ELISA Kit
QY-E21001 96T
EUR 433.2

Human Methylase ELISA Kit
QY-E02921 96T
EUR 448.8

Human Methylase ELISA Kit
YLA2462HU-48T 48T
EUR 435

Human Methylase ELISA Kit
YLA2462HU-96T 96T
EUR 562.5

Mouse Methylase ELISA Kit 　 　 　
YLA0768MO-48T 48T
EUR 465

Mouse Methylase ELISA Kit 　 　 　
YLA0768MO-96T 96T
EUR 600

Mouse Methylase ELISA Kit 　
SL0378Mo -
EUR 528

Human Methylase ELISA Kit
SL1181Hu 96 Tests
EUR 468

TES (N-Tris[hydroxymethyl]methyl-2-Aminoethanesulfonic Acid) Sodium Salt
42020096-1 25 g
EUR 72.29

TES (N-Tris[hydroxymethyl]methyl-2-Aminoethanesulfonic Acid) Sodium Salt
42020096-2 100 g
EUR 136.34

TES (N-Tris[hydroxymethyl]methyl-2-Aminoethanesulfonic Acid) Sodium Salt
42020096-3 500 g
EUR 465.77

Luxol Fast Blue in Acidified Methanol
RRSP248-D 500ml
EUR 14.76

Luxol Fast Blue in Acidified Methanol
RRSP248-E 1L
EUR 22.73

Luxol Fast Blue in Acidified Methanol
RRSP248-F 2.5L
EUR 33.89

Rabbit Methylase ELISA Kit
ERTM0270 96Tests
EUR 625.2

Rabbit Methylase ELISA kit
E04M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Methylase ELISA kit
E04M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Methylase ELISA kit
E04M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Methylase ELISA kit
E09M0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Methylase ELISA kit
E09M0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Methylase ELISA kit
E09M0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Methylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bovine Methylase ELISA Kit
EBM0270 96Tests
EUR 625.2

Canine Methylase ELISA Kit
ECM0270 96Tests
EUR 625.2

Epizen DNA Methylation Kit
Z4201-050 50 Rxns
EUR 392.4

Epizen DNA Methylation Kit
Z4201-200 4x50 rxns
EUR 1161.6

3-Nitro-4-((tetrahydro-2h-pyran-4-yl)methylamino)benzenesulfonamide
20-abx184585
  • EUR 1095.60
  • EUR 2715.60
  • 1 g
  • 5 g

Eva QPCR SuperMix Kit
K5052200 200 reactions
EUR 188

Eva QPCR SuperMix Kit
K5052400 400 reactions
EUR 316

Pro QPCR SuperMix Kit
K5053200 200 reactions
EUR 161

Pro QPCR SuperMix Kit
K5053400 400 reactions
EUR 291

Porcine Methylase ELISA Kit
EPM0270 96Tests
EUR 625.2

ELISA kit for Mouse Methylase
KTE70950-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Mouse Methylase in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Our work thus provides the likelihood to diagnose protothecosis within the samples, the place bacterial mastitis is essentially the most generally presumed and thereby aiding ample corrective measures to be taken.

 

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